Picture from Warren CE et al. (2002) J Biol Chem "The Caenorhabditis elegans gene, gly-2, can rescue the ...."

Figure 8. Pattern of gly-2promoter-directed GFP expression during nematode development. Except for panels F and G, images are presented as pairs showing the epifluorescence from the cytosolic GFP reporter in the upper section and differential interference contrast images from the same field and focal plane in the lower section. The emerald-green light is emitted by GFP, the yellow-green signal is gut autofluorescence. Scale bar in all cases is 20 μm.A, comma stage embryo. B, 1.5-fold stage embryo.C, 3-fold stage embryo. D, lateral view of an early larval stage 4 hermaphrodite peri-vulval region. DNC, dorsal nerve cord; VNC, ventral nerve cord; vul, vulval cells vul E and/or vul F. E, ventral view of late larval stage 4 hermaphrodite peri-vulval region. SV, spermathecal valve; v/u, vulval and/or uterine structures.F, as E, but from a nuclear-localized reporter.G, detail in lateral view of the spermathecal valve staining by the nuclear-localized reporter expressed in an adult hermaphrodite.H, lateral view of an adult hermaphrodite mid-body showing continued expression in the spermathecal valve (SV) and vulval and/or uterine structures (v/u). I and J, lateral views in two different focal planes from adult hermaphrodites showing vulval/uterine staining (v/u).K, lateral view of the pharynx from an adult hermaphrodite showing GFP fluorescence from neurons (N) in the nerve ring and ventral nerve chord (VNC) and the staining of the pharyngeal-intestinal valve (PIV).

Picture from Miyara A et al. (2011) PLoS Genet "Novel and conserved protein macoilin is required for diverse neuronal functions ...."

Figure S4 Subcellular localization of yellow fluorescent protein (YFP)::MACO-1, YFP::MACO-1 TM, and YFP::MACO-1 CC. (A-C) Expression of YFP::MACO-1 in almost all neurons and cyan fluorescent protein (CFP)::TRAM (rER marker) in AFD neurons of maco-1(nj34) animals. This transgenic strain showed a partial-rescued phenotype (Figure S2C). (A) YFP::MACO-1. (B) CFP::TRAM. (C) Merged YFP::MACO-1 and CFP::TRAM images. YFP::MACO-1 was localized to peri-nuclear regions and co-localized with CFP::TRAM, suggesting that MACO-1 is localized to the rER. (D-F) Expression of YFP::MACO-1 TM and CFP::TRAM in AFD neuron of wild-type animals. (D) YFP::MACO-1 TM. (E) CFP::TRAM. (F) Merged YFP::MACO- 1 TM and CFP::TRAM images. YFP::MACO-1 TM was colocalized with CFP::TRAM, suggesting that MACO-1 TM is localized to the rER. (G-I) Expression of YFP::MACO-1 CC and CFP::TRAM in AFD neuron among wild-type animals. (G) YFP::MACO-1 CC. (H) CFP::TRAM. (I) Merged YFP::MACO-1 CC and CFP::TRAM images. YFP::MACO-1 CC was negligibly co-localized with CFP::TRAM, suggesting that MACO-1 CC is only slightly localized to the rER. (J, K) Expression of YFP::MACO-1 TM in almost all neurons in maco-1(nj34). This strain did not show the rescued phenotype (Figure S2C). (J) Merged DIC and YFP images. (K) YFP image. (L, M) Expression of YFP::MACO-1 CC in almost all neurons in maco-1(nj34). This strain showed little to no rescued phenotype (Figure S2C). (L) Merged DIC and YFP images. (M) YFP image. Dashed ellipse shows a nerve ring (i.e., ring-shaped zone where axons of many neurons overlap). YFP::MACO-1 CC is localized to not only cytoplasm but neurites. Adult animals were observed in all images. Scale bars: A-C and J-M= 5 um; D-I= 2 um.

Picture from Taferner A et al. (2015) PLoS One "FAH domain containing protein 1 (FAHD-1) is required for mitochondrial function ...."

Fig 2. Expression and subcellular localization of FAHD-1 in nematodes. (A) Young adult and gravid adult hermaphrodites of the transcriptional reporter N2;Ex[pfahd-1GFP, pRF4] were monitored by confocal microscopy. fahd-1 seems to be expressed in most tissues, including pharynx, canal cell, neurons, vulva, intestine, and stomato-intestinal muscle. Representative pictures are shown. Size bar = 100 um. (B) Confocal images of young adult C. elegans of the transcriptional reporter N2;Ex[pfahd-1GFP, pRF4]. Representative images are shown. Size bar = 10 um. Upper left panel: Detailed picture of fahd-1 expression in the peri-vulvar region; vulva and uterine muscles are stained positive. Upper right panel: Detailed picture of fahd-1 expression in the pharyngeal region; buccal cavity, procorpus, metacorpus, isthmus, posterior bulb, and pharyngeal-intestinal valve, as well as sensory neurons in the head were stained positive. Lower left panel: Detailed picture of fahd-1 expression in the mid-body region; body wall muscles, lateral ganglion, dorsal chord (DC), and ventral nerve chord (VNC) were stained positive. Lower right panel: Detailed picture of fahd-1 expression in the anus region; the stomato-intestinal muscles, the anus and the intestine were stained positive. (C) Temporal expression pattern of fahd-1. The translational reporter N2;Ex[pfahd-1FAHD-1::GFP, pRF4] was examined (and pictured) by confocal fluorescence microscopy from the time point the eggs were laid at gastrula stage to 10-day old post fertile adults. GFP fluorescence was overlaid with phase-contrast microscopy. The FAHD1-1::GFP fusion protein is present in all stages from gastrulation to old worms. Representative images are shown. Size bar = 50 um. (D) Young adult hermaphrodites of the translational reporter N2;Ex[pfahd-1FAHD-1::GFP, pRF4] expressing the FAHD-1::GFP fusion protein (shown in green) were stained with 100 nM MitoTracker Red (shown in red) overnight. Shown here are confocal pictures of the pharynx region of the worm recorded in the green and red channel, as well as the merged pictures. Regions of overlapping staining (shown in yellow) are indicated by arrows. Representative pictures are shown. Size bar = 10 um.