Figure 1.
che-5(
e1073) carries mutations in
gcy-22 that are responsible for chemotaxis defects of the mutants: (A) Schematic diagram of
gcy-22a gene structure. Boxes represent exons. Protein domains and the nucleotide substitutions found in
e1073 are indicated above and below the diagram, respectively. (B) Complementation test between
che-5(
e1073) and
gcy-22(
tm2364). Salt concentration chemotaxis was observed in
e1073/tm2364 heterozygotes and their parental strains.
e1073 failed to complement
tm2364. n = 5, Mean+/-SEM, *p < 0.001, compared to mIs11, Dunnett's test. (C) ASER-specific expression of
gcy-22(+) rescued the chemotaxis defect of the
che-5(
e1073) mutants. n = 4 or 5, Mean+/-SEM, *p < 0.01, compared to wild type, Dunnett's test.