Figure 7. RIC-8 Is Concentrated in the Nervous System of Adult Animals. (A) Head region of a wild-type C. elegans adult stained with an antibody to RIC-8. Note the prominent staining of the head ganglia, amphid process, and ventral nerve cord.(B) Close-up view of a section of the head ganglia. White arrowheads indicate examples of several strongly stained neurons in which RIC-8 immunoreactivity is present throughout the cytoplasm of the cell. Note the variability in RIC-8 staining intensity between different neurons.(C) RIC-8 is also present at some cholinergic synapses in the ventral cord. Close-up view of a region near the vulva that was double stained with RIC-8 (green staining) and CHA-1 (red staining), a marker that is localized to cholinergic synapses (represented by bright puncta) (Janet Duerr, personal communication). Regions of overlap show up as yellow. Arrowheads indicate a subset of synapses along the ventral nerve cord that are positive for both RIC-8 and CHA-1.(D) Expanded view of a region of head ganglia showing RIC-8 staining in the nerve ring, which contains axonal processes.(E) Head region of a
ric-8(
md1909) adult stained with an antibody to RIC-8. Note that the nervous system staining is decreased, though not absent, in this transposon insertion mutant.