Picture from Kuhara A et al. (2008) Science "Temperature sensing by an olfactory neuron in a circuit controlling behavior of ...."

Figure S2. Expression pattern of eat-16 and promoters for rescue experiments. (A) Cells expressing eat-16::gfp in wild-type. Yellow bar indicates 10 micrometers. (B) List of cells expressing GFP fluorescence driven by fusion genes encoding the indicated promoters and gfp gene (promoter::gfp). The eat-4, odr-3, gpa-13, odr-1 promoters all drive gene expression in AWC, although these promoters drive gene expression in different sets of neurons.

Picture from Kuhara A et al. (2008) Science "Temperature sensing by an olfactory neuron in a circuit controlling behavior of ...."

Cells expressing eat-16::gfp in wild-type. Yellow bar indicates 10 micrometers.

Picture from Kuhara A et al. (2002) Neuron "Negative regulation and gain control of sensory neurons by the C. elegans ...."

(D-I) GFP expression of pAK43 tax-6::GFP. GFP fluorescence was detected in many sensory neurons and interneurons near the nerve ring in the head (D-F), body wall muscle (G), vulval muscle, as indicated by white arrow (H), and pharyngeal muscle, as indicated by white arrow (I). TAX-6::GFP seems to be localized near the dense body or M line in body wall muscles, although TAX-6::GFP expression was clearly detected in the whole body wall muscle cell (G). Shown here are left-lateral views of adult animals (G-I), L2 larvae (D and E), and an L1 larva (F). Anterior is to the left, and dorsal is up (D-G). A ventral view is shown in (H), where the fluorescence was undetectable in one of the muscle cells (indicated by hatched arrow) probably due to mosaicism. Scale bar: 10 μm.

Picture from Kuhara A et al. (2002) Neuron "Negative regulation and gain control of sensory neurons by the C. elegans ...."

(A) tax-6 expression was detected in many neurons and muscles. Introduction of four GFP fusion constructs into wild-type or tax-6 animals revealed three distinct regions that drive tax-6 expressions in different cell types. Since particular attention was made to identify amphid sensory organ-related sensory neurons and interneurons expressing tax-6, the cells listed here do not represent all cells with the GFP fluorescence.(B) Summary of amphid-associated neurons expressing tax-6 and their functions.

Picture from Kuhara A et al. (2002) Neuron "Negative regulation and gain control of sensory neurons by the C. elegans ...."

(J and K) GFP expression of pAK13 tax-6::GFP. TAX-6::GFP was observed in the entire cytoplasm of head sensory neurons including the sensory cilia situated at tip of the head. Shown here are left-lateral views of adult animals. Anterior is to the left, and dorsal is up. Nomarski (gray) and GFP (orange) views were merged in (K). Scale bar: 10 μm.

Picture from Kuhara A et al. (2002) Neuron "Negative regulation and gain control of sensory neurons by the C. elegans ...."

Figure 3. The Expression Analysis of tax-6. (A) tax-6 expression was detected in many neurons and muscles. Introduction of four GFP fusion constructs into wild-type or tax-6 animals revealed three distinct regions that drive tax-6 expressions in different cell types. Since particular attention was made to identify amphid sensory organ-related sensory neurons and interneurons expressing tax-6, the cells listed here do not represent all cells with the GFP fluorescence.(B) Summary of amphid-associated neurons expressing tax-6 and their functions.(C) The thermosensory circuit (Mori and Ohshima, 1995). Temperature inputs are sensed by the AFD and unidentified sensory neuron(s), as indicated by the letter X. Thermal information is assumed to be integrated by cross-talk between AIY and AIZ interneurons. The AFD-AIY arm of the circuit mediates movement to higher temperatures (as indicated by the letter T), while the AIZ arm mediates movement to lower temperatures (as indicated by the letter C).(D-K) GFP expression of pAK43 tax-6::GFP (D-I) and pAK13 tax-6::GFP (J and K) (see Figure 2A). GFP fluorescence was detected in many sensory neurons and interneurons near the nerve ring in the head (D-F), body wall muscle (G), vulval muscle, as indicated by white arrow (H), and pharyngeal muscle, as indicated by white arrow (I). TAX-6::GFP seems to be localized near the dense body or M line in body wall muscles, although TAX-6::GFP expression was clearly detected in the whole body wall muscle cell (G). TAX-6::GFP was observed in the entire cytoplasm of head sensory neurons including the sensory cilia situated at tip of the head (J and K). Shown here are left-lateral views of adult animals (G-K), L2 larvae (D and E), and an L1 larva (F). Anterior is to the left, and dorsal is up (D-G and I-K). A ventral view is shown in (H), where the fluorescence was undetectable in one of the muscle cells (indicated by hatched arrow) probably due to mosaicism. Nomarski (gray) and GFP (orange) views were merged in (K). Scale bar: 10 μm.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.