Picture from Labrousse AM et al. (1998) Mol Biol Cell "Contribution of the GTPase domain to the subcellular localization of dynamin in ...."

Figure 1. Endogenous dynamin detected in C. elegans by immunofluorescence. A wild-type (N2) animal was stained with anti-dynamin antibodies. The immunofluorescence is concentrated in the nerve ring (nr) and along ventral (vc) and dorsal (dc) nerve cords. The punctate pattern in the pharynx corresponds to pharyngeal neurons (pn). Dynamin was also detected in sublateral neurons (sn). The inset shows that dynamin accumulates along the apical surface of intestinal cells (arrowhead). In this case, the worm was broken during the permeabilization procedure, which helped unmask the specific intestinal staining.

Picture from Lyssenko NN et al. (2008) BMC Dev Biol "An unexpectedly high degree of specialization and a widespread involvement in ...."

Figure 5. tat-3 reporter expression pattern. The 5' end of the tat-3 locus, location of the deleted region in tm1275 mutants (bar) and structure of the tat-3 expression cassette (A). tat-3 reporter expression in the pharyngeal-intestinal valve and in muscle and marginal, but not gland, cells of the pharynx in the lcIs471.12 line (B). GFP fluorescence in the muscle and buccal epithelial cells of the pharynx procorpus and in the XXX cells in the lcIs471.4B line (C). tat-3 reporter signals in the rectum and a tail region (D), seam cells and the hypodermis (E), the DTC (F) and the AC (G) of lcIs471.4B animals. GFP staining of the vulva at the late L4 stage in lcIs881.3.2 larvae (H). Reporter expression in the adult lcIs471.4B vulva (I). Cell labels: AC - anchor cell; DTC - distal tip cell; e1 and 2 - buccal epithelial cells; g1 - gland cell; mc - marginal cell; pm2, 5, 6 and 7 - pharyngeal muscle cells; vul - vulval cells; utse - uterine seam cell; XXX - XXX cells. Abbreviations: hn - hypodermal nucleus; pv - pharyngeal-intestinal valve; sn - seam cell nucleus.

Picture from Diaz-Balzac CA et al. (2016) Curr Biol "Muscle- and Skin-Derived Cues Jointly Orchestrate Patterning of Somatosensory ...."

(C) Schematic of the CRISPR/Cas9 modifiedlect-2 locus and images of LECT-2::mNeonGreenexpression at endogenous levels during differentembryonic stages.(D and E) Fluorescent micrographs of adultanimals of the CRISPR/Cas9 modified LECT-2::mNeonGreen strain showing LECT-2::mNeonGreenlocalization at endogenous levels. Staining is seenon processes of the nervous system, including theventral nerve cord (vnc), dendrites of sensoryneurons (sn), and coelomocytes (cc; see theenlarged panel on the right with vesicular staining). The nerve ring also shows staining(D, magenta arrowhead), but the associated headneurons, located immediately posterior to thenerve ring, show little or no staining. Expression isalso seen surrounding the seam cells (sc), lateralhypodermal ridge (E, white arrowheads), motorneurons (see Figure 4G) and associated commissures (co), the ventral nerve cord (vnc), theALM mechanosensory neuron (D, empty arrowhead), and the basement membrane of body-wallmuscles (mbm) and the parallel bands of fibrousorganelles (fo) (E, inset). Only weak, if any, stainingwas seen on PVD neurons.(F) Micrographs of the SAX-7::GFP fosmid reporter [ddIs290] showing expression in the samelocations as LECT-2::mNeonGreen, including theseam cells (sc), lateral hypodermal ridge (whitearrowhead), commissures (co), and the ALMneuron (empty arrowhead). As with the translational LECT-2 reporter shown in (B), expressionalong the lateral hypodermal ridge co-localizeswith the tertiary branches of PVD, visualizedby the dzIs53 [F49H12.4::mCherry] transgene.Anterior is to the left and dorsal is up; the scale barrepresents 20 um in all panels.

Picture from Diaz-Balzac CA et al. (2016) Curr Biol "Muscle- and Skin-Derived Cues Jointly Orchestrate Patterning of Somatosensory ...."

Figure 2. LECT-2/Chondromodulin II Is Expressed in Muscle and Localizes to the Nervous System and Basement Membranes(A) Schematic of the lect-2 transcriptional reporterand fluorescent micrograph of an adult transgenicanimal carrying the Plect-2::mCherry transgene.Expression is seen in body-wall muscle.(B) Schematic of the LECT-2 translational reporterand maximum-intensity projections of an animalshowing expression of the LECT-2::mCherrytranslational reporter in a lect-2 mutant background. PVD is visualized by the wdIs52[F49H12.4::GFP] transgene. Vesicular coelomocyte (cc) expression is shown in a separate panel.Expression is seen along the lateral hypodermalridge (empty arrowheads) and continues along thetertiary line of PVD branches (white arrowheads).(C) Schematic of the CRISPR/Cas9 modifiedlect-2 locus and images of LECT-2::mNeonGreenexpression at endogenous levels during differentembryonic stages.(D and E) Fluorescent micrographs of adultanimals of the CRISPR/Cas9 modified LECT-2::mNeonGreen strain showing LECT-2::mNeonGreenlocalization at endogenous levels. Staining is seenon processes of the nervous system, including theventral nerve cord (vnc), dendrites of sensoryneurons (sn), and coelomocytes (cc; see theenlarged panel on the right with vesicular staining). The nerve ring also shows staining(D, magenta arrowhead), but the associated headneurons, located immediately posterior to thenerve ring, show little or no staining. Expression isalso seen surrounding the seam cells (sc), lateralhypodermal ridge (E, white arrowheads), motorneurons (see Figure 4G) and associated commissures (co), the ventral nerve cord (vnc), theALM mechanosensory neuron (D, empty arrowhead), and the basement membrane of body-wallmuscles (mbm) and the parallel bands of fibrousorganelles (fo) (E, inset). Only weak, if any, stainingwas seen on PVD neurons.(F) Micrographs of the SAX-7::GFP fosmid reporter [ddIs290] showing expression in the samelocations as LECT-2::mNeonGreen, including theseam cells (sc), lateral hypodermal ridge (whitearrowhead), commissures (co), and the ALMneuron (empty arrowhead). As with the translational LECT-2 reporter shown in (B), expressionalong the lateral hypodermal ridge co-localizeswith the tertiary branches of PVD, visualizedby the dzIs53 [F49H12.4::mCherry] transgene.Anterior is to the left and dorsal is up; the scale barrepresents 20 um in all panels.