Picture from Hobert O et al. (1999) J Cell Biol "A conserved LIM protein that affects muscular adherens junction integrity and ...."

Figure 7. unc-97 functions in muscle development. (A-C): UNC-97::GFP localization in live worms at embryonic stage 300 min (A), 400 min (comma stage) (B), and 500 min (threefold pretzel stage) (C). (A'-C') Corresponding DIC micrographs. White arrows in C, a muscle nucleus and dense bodies, respectively. Note UNC-97::GFP localization to the periphery of nuclei in A-C. (D-F): Loss-of-function phenotype of unc-97 as determined by RNAi. D and E show the progeny of animals subjected to unc-97 RNAi. Embryos either arrest and die at the twofold stage or die shortly after hatching (both categories were scored as arrested). Note that the pharynx develops relatively normally but that elongation of the rest of the animal is arrested. All these phenotypes are hallmarks of the pat phenotype (Williams and Waterston, 1994). Bottom, progeny of animals subject to RNAi with a control ds RNA derived from a new homeobox gene (Hobert, O., and G. Ruvkun, unpublished data), which displays no lethality.

Picture from Liu Q et al. (2010) J Cell Sci "Facioscapulohumeral muscular dystrophy region gene-1 (FRG-1) is an ...."

Figure 4. FRG-1 associates with body-wall muscle cell dense bodies, adjacent to PAT-3, and colocalizes with ALP-1. (A-F) Adult animals co-immunostained for FRG-1 (A,D, green) and PAT-3 (B,E, red) show FRG-1 present at all body-wall muscle dense bodies (C, arrowheads), whereas it is absent from M-lines (C, arrows). FRG-1 and PAT-3 show adjacent, partially overlapping signals at dense bodies when merged (C,C', arrowheads). (D-F) Confocal images of dense body immunostaining. (G-I) Double transgenic animals for Pmyo-3::frg-1::yfp and Pmyo-3::alp-1alpha::cfp show that expression patterns for FRG-1 (G) and ALP-1 (H) are precisely colocalized in the image merge (I and I'). (J-L) Mutant C. elegans strain containing a homozygous partial deletion for atn-1 was co-immunostained for FRG-1 (J, green) and PAT-3 (K, red), showing disrupted FRG-1 localization (L). (M) Model depicting FRG-1 localization in dense bodies with the analogous vertebrate components (based on Moerman and Williams, 2006; Fluck et al., 2002). Scale bars: 20 μm. DGC, dystrophin-glycoprotein complex; IRC, integrin receptor complex.

Picture from McKeown CR et al. (1998) Development "sma-1 encodes a betaH-spectrin homolog required for Caenorhabditis elegans ...."

Figure 9. Location of sma-1 RNA in C. elegans embryos by whole-mount in situ hybridization. Embryos were hybridized with pAZ4 DIG-labeled antisense probe. In all pictures anterior is to the left. (A) Early morphogenesis; lateral view, mid-focal plane, dorsal is at top of photograph. sma-1 RNA is present in cells on the posterior dorsal surface of the embryo. (B-D) Early morphogenesis (older than A); dorsal view, multiple focal planes of single embryo. (B) Upper focal plane; sma-1 RNA is present in the cells of the dorsal epidermis. (C) Mid-focal plane; sma-1 RNA is seen in the outermost layer of cells, except in the region of the head. Arrowhead, early sma-1 RNA expression in the intestine. (D) Lower focal plane; sma-1 RNA is seen in a small group of cells on the left and right side of the ventral surface (arrowheads). The position of these sma-1 expressing cells corresponds to that of the leading cells' (Williams-Masson et al., 1997), which initiate ventralward migration of the epidermis and are the first epidermal cells to reach the ventral surface. (E-H) Lateral view; mid-focal plane, dorsal is at top of photograph. By lima bean stage (E), sma-1 RNA is present on the ventral surface of the embryo (arrowhead); by comma stage (F), sma-1 RNA is present on the anterior surface of the embryo (arrowhead). sma-1 expression in pharynx and intestine is most apparent in 1.75-fold stage embryos (G); arrow marks location of boundary between pharynx and intestine. At the 2.5-fold stage (H) sma-1 RNA is not detected in the epidermis, but is still present in the intestine. Additional sma-1 expression is seen in the excretory cell (white arrowhead) and an unidentified cell near the anus (white arrow). Bar in H is 10 µm.