Picture from Belfiore M et al. (2002) RNA "The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene ...."

A: Northern analysis of mep-1 mRNA. Three micrograms of poly(A)-enriched RNA were loaded per lane. Lanes 1 to 6 contain RNA from wild-type worms at different developmental stages (embryos; larval stages L1 to L4, adults). Lane 7 contains RNA from glp-1(q224) adult worms that contain virtually no germline. Lane 8 corresponds to RNA from wild-type worms at mixed developmental stages. The same blot was probed with actin mRNA (act-1, a gift from M. Krause) as a loading control.

Picture from Yuan JY et al. (1993) Cell "The C. elegans cell death gene ced-3 encodes a protein similar to mammalian ...."

Figure 2. ted-3 RNA Is Expressed in Embryos.A Northern blot of poly(A)+ RNA from mixed stages, embryos, Ll-L4 larvae, and young adults, probed with the ted-3 cDNA subclone pJl18 (see Experimental Procedures). pJ118 did not detectably hybridize to RNA derived from glpl(q237) adults (which lack a germline; Austin and Kimble, 1987) (data not shown), suggesting that the ted-3 RNA detected in the young adults in this experiment was derived from em- bryos within these animals. The level of RNA in each lane can be estimated based upon hybridization to a control actin 1 probe (Krause and Hirsh, 1984).

Picture from Yuan JY et al. (1992) Development "The Caenorhabditis elegans cell death gene ced-4 encodes a novel protein and is ...."

Figure 3. The ced-4 transcript is expressed mostly during embryogenesis. (a) A northern blot of poly(A)+ RNA from stage-synchronized animals was probed using pn1416, which hybridizes both to the 2.2 kb ced-4 transcript and to a 0.9 kb transcript (see Fig. 2A). Only the 2.2 kb transcript is shown in this figure. The 0.9 kb transcript is expressed mostly in eggs and adults (data not shown). (b) The presence of RNA in all lanes was confirmed by loading 1/10 of each sample on another gel and probing a northern blot from this gel using the C. elegans actin 1 gene (Krause and Hirsh, 1984).

Picture from Rosenquist TA et al. (1988) Genes Dev "Molecular cloning and transcript analysis of fem-3, a sex-determination gene in ...."

Developmental profile of fem-3 transcripts. Poly(A)+ RNAs were isolated from developmentally staged hermaphrodites and were separated by electrophoresis in a 2.0% denaturing agarose gel and transferred to nitrocellulose. The filter was hybridized to a fem-3 probe (A) (cDNA clone pJK50, see Materials and methods) and then rehybridized to an act-1 probe (B). The amount of act-1 RNA in total RNA appears to be constant throughout postembryonic development (Krause and Hirsh 1984); therefore, the act-1 signal serves as an internal control for the amount of RNA loaded. Arrows indicate fern-3 transcripts. (A and B) (Lane E) Embryonic RNA; (lanes L1-L4) RNAs from first through fourth larval stages, respectively; (lane A I Adult RNA).

Picture from Spike CA et al. (2002) Development "MEC-8 regulates alternative splicing of unc-52 transcripts in C. elegans ...."

Figure 1. MEC-8 expression pattern. (A,C) Early embryos with anti-MEC-8 staining in all nuclei. (B) DAPI staining of the embryo shown in A. (D,E) Older embryos with anti-MEC-8 staining in hypodermal nuclei. (F) A lower focal plane of the same embryo as in E. This embryo was transgenic for pPD37.48, an hlh-1::lacZ fusion construct (Krause et al., 1994); muscle cells stained with anti-β-galactosidase and not with anti-MEC-8. (G) MEC-8::GFP in a 1.5-fold embryo. (H) Anti-LIN-26 hypodermal staining of the embryo shown in G, showing overlapping staining with anti-MEC-8. (I) A mec-8(u391) embryo lacking anti-MEC-8 nuclear stain. (J) The mid-body region of an L1 larva in which hyp7 nuclei were stained with anti-MEC-8. (K) DAPI stain of the larva shown in J. (L) The mid-body region of an adult stained with anti-MEC-8. The white arrow indicates ALML (touch neuron), and the black arrow indicates the vulva. Scale bar: 10 μm.

Picture from Solari F et al. (1999) Development "The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of ...."

Figure 1. Characterisation of egl-27(we3) and egl-27::gfp expression. (A) vab-7(ed6) L1 hermaphrodite is nearly identical to wild type except for a small bulge at the tip of the tail (arrow), where the wild-type tail is sharply pointed. (B) egl-27(we3) L1 grown at 15 C. (C-F) Muscle patterning in wt (C,D) and egl-27(we3) (E,F) embryos at 1.5-fold stage of development. Muscle cells in D and F are visualised using hlh-1::gfp, a reporter for the C. elegans MyoD homolog (Krause et al., 1990; K. Dej, S. Xu, and A. Fire personal communication); (C,E) DIC images of the same embryos. In D, two of the four muscle rows are visible in this focal plane. Arrow in F points to a cluster of muscle cells. (G,H) Seam patterning in wt (G) and egl-27(we3) (H), visualised using antibody NE2/1B4.14; arrowsin H shows forked and disrupted posterior seam. (I) L1 larvae induced by egl-27(exon 11) RNAi. (J,K) egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene. Scale bar in B is for B,I; scale bar in C is for C-H and J.

Picture from Mathies LD et al. (2003) Development "The C. elegans Hand gene controls embryogenesis and early ...."

Figure 6. hnd-1::GFP expression. (A) Lineage diagram depicting cells that express hnd-1::GFP (green). Dashed lines indicate approximate stage of embryos in panels B-D. (B-H) Confocal images of embryos expressing hnd-1::GFP (green). (B-D) Projections of z-series; embryos were observed over time to identify cells. (B) hnd-1(N)::GFP expression is first detected in four granddaughters of MS, descendants of MS.ap and MS.pp, in four granddaughters of C, descendants of C.ap and C.pp (C.xp), and in two daughters of D. (C) hnd-1(FL)::GFP expression in granddaughters of MS.ap/MS.pp and daughters of C.ap/C.pp. (D) hnd-1(N)::GFP expression fades after the next division of most hnd-1-expressing cells but is retained in daughters of MS.appp and MS.pppp, and in daughters of C.ppp and C.app. (E-H) Embryos fixed and stained with α-HLH-1 or α-PGL-1 (red). (E) Unlike HLH-1 (red), which is detected in body muscle lineages throughout embryogenesis (Krause et al., 1990), hnd-1(N)::GFP is absent from body muscles by the comma-stage of embryogenesis; at this time, expression is seen in Z1/Z4. (F) hnd-1(N)::GFP is detected in Z1 and Z4 as they meet the PGCs (Z2, Z3), marked by PGL-1 (red). (G) Shortly after, expression is absent from Z1 and Z4. (H) hnd-1 embryos express hnd-1(N)::GFP in Z1/Z4.

Picture from Belfiore M et al. (2002) RNA "The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene ...."

FIGURE 4. Expression of mep-1 RNA and protein. A: Northern analysis of mep-1 mRNA. Three micrograms of poly(A)-enriched RNA were loaded per lane. Lanes 1 to 6 contain RNA from wild-type worms at different developmental stages (embryos; larval stages L1 to L4, adults). Lane 7 contains RNA from glp-1(q224) adult worms that contain virtually no germline. Lane 8 corresponds to RNA from wild-type worms at mixed developmental stages. The same blot was probed with actin mRNA (act-1, a gift from M. Krause) as a loading control. B-E: Expression of mep-1::GFP in somatic tissues. GFP was observed in most nuclei of embryos (B) and L1 larvae (C), but was only occasionally ob- served in adult worms (D, head; E, nuclei of the hypodermis). F: Immunostaining with anti-MEP-1 antibodies in the germline. The black arrowhead shows a nucleus in the distal arm of the gonad. No staining is observed in the proximal region of the germline (white bracket). G: Nuclear DAPI staining of the animal in F. The bracket shows the region containing mature sperm (white arrowhead) and sperm precursors (white arrow) that have condensed nuclei. F and G are in the same focal plane and represent one gonadal arm of an L4 larva that has not started oogenesis. The bar repre- sents 20 um Anterior is to the left in C to G.

WBPicture0000004549

Diffuse mosaic expression is seen in the pharynx from the L2 larval stage to adult. This expression is possibly spurious (Hope, I.A. (1991). Development, 113, 399. Krause, M. et al. (1994). Dev. Biol., 166, 133.). However, the upstream region used in this construct is 5.7kb and should contain all upstream promoter elements.

WBPicture0000004550

Diffuse mosaic expression is seen in the pharynx from the L2 larval stage to adult. This expression is possibly spurious (Hope, I.A. (1991). Development, 113, 399. Krause, M. et al. (1994). Dev. Biol., 166, 133.). However, the upstream region used in this construct is 5.7kb and should contain all upstream promoter elements.