(A-F) The wild-type background (strain DG4190).(G and H) The
lin-41(
n2914) null background(from strain DG4206). (I and J) An
oma-1;
oma2 null background (from strain DG4253). (K andL) A
gld-2(
q497) null background (from strainDG4242). GFP::3xFLAG::CDC-25.3 expressionwas detected by (B and D) GFP fluorescence or(F, H, J, and L) indirect immunofluoresence indissected gonads stained with anti-FLAG antibody.