Picture from Koelle MR et al. (1996) Cell "EGL-10 regulates G protein signaling in the C. elegans nervous system and shares a ...."

EGL-10 Protein Expression. (A) Western blot analysis of protein extracts from wild-type and egl-10(md176) worms probed with the affinity-purified anti-EGL-10 polyclonal antibodies. The closed arrow indicates the position of the EGL-10 protein detected in wild-type but not in egl-10 mutant extracts. The open arrow indicates the 47 kDa protein that cross-reacted with the EGL-10 antibodies but was not a product of the EGL-10 gene. The positions of molecular weight markers are indicated, with their sizes in kilodaltons.(B) Anti-EGL-10 antibody staining of the head of a wild-type adult hermaphrodite. The dark immunoperoxidase stain labeled the neural processes of the nerve ring (arrow).(C) Anti-EGL-10 antibody staining of the head of an egl-10(md176) adult hermaphrodite, prepared in parallel to (B) and lacking any specific staining.(D) Anti-EGL-10 immunofluorescence staining in the midbody region of a wild-type adult. The fluorescence here and in (E) appears white on a black background, the reverse of the staining in (B) and (C). The arrow points to the brightly stained ventral cord neural processes. Body-wall muscle cells on either side of the ventral cord contained brightly stained spots arranged in linear arrays. Body-wall muscles throughout the animal showed similar staining.(E) Fluorescence in the head of a transgenic adult carrying a fusion of the egl-10 promoter and N-terminal coding sequences to the GFP gene. The fusion protein is localized in spots within the body-wall muscles similar to those seen in (D). GFP fluorescence was also present in neural processes and cell bodies out of the plane of focus.

Picture from Koelle MR et al. (1996) Cell "EGL-10 regulates G protein signaling in the C. elegans nervous system and shares a ...."

Figure 3. EGL-10 Protein Expression. (A) Western blot analysis of protein extracts from wild-type and egl-10(md176) worms probed with the affinity-purified anti-EGL-10 polyclonal antibodies. The closed arrow indicates the position of the EGL-10 protein detected in wild-type but not in egl-10 mutant extracts. The open arrow indicates the 47 kDa protein that cross-reacted with the EGL-10 antibodies but was not a product of the EGL-10 gene. The positions of molecular weight markers are indicated, with their sizes in kilodaltons.(B) Anti-EGL-10 antibody staining of the head of a wild-type adult hermaphrodite. The dark immunoperoxidase stain labeled the neural processes of the nerve ring (arrow).(C) Anti-EGL-10 antibody staining of the head of an egl-10(md176) adult hermaphrodite, prepared in parallel to (B) and lacking any specific staining.(D) Anti-EGL-10 immunofluorescence staining in the midbody region of a wild-type adult. The fluorescence here and in (E)-(G) appears white on a black background, the reverse of the staining in (B) and (C). The arrow points to the brightly stained ventral cord neural processes. Body-wall muscle cells on either side of the ventral cord contained brightly stained spots arranged in linear arrays. Body-wall muscles throughout the animal showed similar staining.(E) Fluorescence in the head of a transgenic adult carrying a fusion of the egl-10 promoter and N-terminal coding sequences to the GFP gene. The fusion protein is localized in spots within the body-wall muscles similar to those seen in (D). GFP fluorescence was also present in neural processes and cell bodies out of the plane of focus.(F) Anti-EGL-10 antibody staining in the head of a transgenic worm carrying the nIs51 multicopy array of wild-type egl-10 genes. The processes of the nerve ring were visible, as in (B), but overexpression of EGL-10 caused the protein also to be detected in cell bodies.(G) Anti-EGL-10 antibody staining in the vulva region of nIs51 worms. The open arrow points to the vulva. The large closed arrow indicates the HSN neuron. The small closed arrow points to the ventral cord and associated neural cell bodies.