Picture from Klein RD et al. (1993) Cell "Independent domains of the Sdc-3 protein control sex determination and dosage ...."

Figure 3. Developmental Regulation of sdc-3 Transcript LevelsUsing a Northern blot analysis, poly(A)+ RNAs (10 ug per lane) isolated from either eggs, the four larval stages, or nongravid adults were hybridized with a 9.5 kb sdc-3 probe from pTY74 (top). To establish that similar amounts of poly(A)+ RNA from each stage had been loaded in all lanes, we stripped and reprobed the blot with an actin probe specific to act-I (bottom).