Picture from Kim JS et al. (2010) Development "C. elegans STRADalpha and SAD cooperatively regulate neuronal polarity and ...."

(A) The expression of a Pstrd-1-nls::gfp reporter in the pharynx, excretory canal and the entire nervous system. (D) Sub-cellular localization of GFP-tagged, functional STRD-1 in GABAergic neurons. GFP::STRD-1 localized to the cytoplasm and the nucleus as well as along both nerve cords. Scale bars: 5 μm.

Picture from Kim JS et al. (2010) Development "C. elegans STRADalpha and SAD cooperatively regulate neuronal polarity and ...."

Fig. 3. Pseudokinase STRD-1 functions cell- autonomously in neurons. (A)The expression of a Pstrd-1-nls::gfp reporter in the pharynx, excretory canal and the entire nervous system. (B)Cell-autonomous rescue of the neuronal polarity defect in strd-1 mutants. The VD polarity defect in strd-1(rr91) mutant animals was fully rescued by the expression of STRD-1 solely in GABAergic neurons under Punc-25. STRD-1(D175A) also fully rescued the VD polarity defect. Values are means ± s.d.; ***, P<0.001; N.S., not significant, Wilcoxon rank- sum test; n≥10 per strain. (C)Cell-autonomous rescue of strd-1 synaptic organization defect. strd-1(ok2283) mutants (i) expressing STRD-1 (ii) or STRD-1(D175A) (iii) in GABAergic neurons showed rescued juIs1 morphology. Distribution of the juIs1 punctum widths showed significantly reduced kurtoses in the rescued animals (P<0.001, strd-1 versus either STRD-1- expressing lines, t-test; n>80 per strain). x-axis, punctum width in pixels; y-axis, density in arbitrary units. (D)Sub-cellular localization of GFP-tagged, functional STRD-1 in GABAergic neurons. GFP::STRD-1 localized to the cytoplasm and the nucleus as well as along both nerve cords. Scale bars: 5um.

Picture from Kim J et al. (2007) Curr Biol "C. elegans CUL-4 prevents rereplication by promoting the nuclear export of CDC-6 ...."

Figure S2. CDC-6::GFP Localization through the Cell Cycle. (A) Dynamic localization of CDC-6::GFP through the cell cycle. Representative pictures of seam cells at the indicated cell-cycle phases are presented. The scale bar represents 10 mm.(B) CDC-6 colocalizes with mitotic chromosomes. Epifluorescence images of CDC-6::tdTomato and histone H1::GFP in a metaphase cell are shown. The scale bar represents 5 mm.(C) Time course of CDC-6 translocation from the nucleus to the cytoplasm in V1-V6 seam cells. A graph of nuclear versus cytoplasmic localization of CDC-6::GFP or CDC-6::tdTomato at times after hatch is shown. No differences in the localization of CDC-6::GFP and CDC-6::tdTomato were observed. Error bars reflect standard error of the mean (SEM). See the Experimental Procedures section for the number of cells analyzed.(D) CDC-6::GFP is translocated to the cytoplasm in the V1-V6 seam cells. Epifluorescence images of CDC-6::GFP in V1-V6 seam cells that are in G1 phase (90-105 min after hatch) or S or G2 phase (225-240 min after hatch). The QR cell and its descendents (QR.a and QR.p) have different cell-cycle timing. The scale bar represents 10 mm.

Picture from Kim SK et al. (2000) Science "Positioning of longitudinal nerves in C. elegans by nidogen."

Figure 4. Nidogen is associated with body wall basement membranes. (A) Larva stained to reveal anti-nidogen antibodies. Intense staining is associated with the basement membrane of the body wall muscle. (B) Muscle cells in the same larva visualized by using an anti-UNC-54 myosin monoclonal antibody. The muscle cells form four sublateral rows extending the length of the body. One muscle quadrant is in the plane of view. Scale bar, 10 um.

Picture from Kim SK et al. (2000) Science "Positioning of longitudinal nerves in C. elegans by nidogen."

Figure 5. Expression of the nid-1 gene detected by using the nid-1 promoter to drive GFP expression. Embryos (A, B, C, D, and E) and larvae (F, G, H, and I). (A and B) Shortly before (A) and at the beginning (B) of embryonic elongation, expression is detected in the Cep (cephalic), IL (inner labial) and OL (outer labial) neurons (arrows). (C, D, and E) During succeeding stages of elongation until hatching, expression is detected in body wall muscle (arrowheads), the intestinal and anal depressor muscle cells (*), and the ALM neurons. (F, G, H, and I) In the larva, expression is detected in the head Ceh, IL, and OL neurons (F), the excretory cannel cell (exc) and anterior intestine (int) (G), the distal tip cells (DTC) and the HSN neurons (H), and posteriorly in the PLM neurons and the anal depressor and intestinal muscle cells (mu int and mu anal) (I).

Picture from Kim KW et al. (2016) BMC Biol "Coordinated inhibition of C/EBP by Tribbles in multiple tissues is essential for ...."

i Fluorescence images of endogenous NIPI-3 expression visualized in the GFP KI strain (fr152). Expression isobserved in the nuclei (yellow arrows) of the epidermis (left panel), the intestine (upper right panel) and the head neurons (lower right panel)

Picture from Kim KW et al. (2016) BMC Biol "Coordinated inhibition of C/EBP by Tribbles in multiple tissues is essential for ...."

Fig. 1 C. elegans Tribbles nipi-3 is required for larval development and viability. a The nipi-3 locus. Top, nipi-3 encodes a pseudokinase of theTribbles family. Middle, nipi-3 deletions generated using CRISPR-Cas9 genome editing. Bottom, the extent of the nipi-3 genomic region used torescue the deletion mutants. b-e Bright-field images of worms at 3 days post-hatching; wild type (b), nipi-3 null mutants (c,d) and a transgenicanimal (Tg) expressing the wild-type nipi-3 genomic DNA in a nipi-3(0) background (e). f Fluorescence images of worms expressing AJM-1::GFPreporter in the epithelial cells to allow visualization of seam cells. g Overlaid differential interference contrast (DIC) and fluorescence image of a nipi3(fr148) mutant at 3 days post-hatching expressing lag-2p::GFP reporter. This image corresponds to the boxed region in d. Two distal tip cells (DTC) areshown in green (arrow), and the germline of arrested nipi-3 null larva is denoted by a dotted red line. h Body length (um) of worms at 3 days posthatching. Each dot represents a single animal measured as shown; each red line represents the mean value. ***P < 0.001; ns, not significant (one-wayANOVA with Tukey's post hoc tests). i Fluorescence images of endogenous NIPI-3 expression visualized in the GFP KI strain (fr152). Expression isobserved in the nuclei (yellow arrows) of the epidermis (left panel), the intestine (upper right panel) and the head neurons (lower right panel)

Picture from Kim KW et al. (2016) BMC Biol "Coordinated inhibition of C/EBP by Tribbles in multiple tissues is essential for ...."

d-j Fluorescence images of cebp-1p::GFP reporter animals at L2. k qRT-PCR analysis of cebp-1 in WT, nipi-3(fr4), nipi-3(0) animals. Relative abundance ofcebp-1 mRNA normalized to actin mRNA. n = 3; error bars represent SEM; *P < 0.05; **P < 0.01; ns, not significant (one-way ANOVA with Tukey'spost hoc tests). l, m Confocal fluorescence images (z-stack) of col-154p(epidermis)::CEBP-1::GFP reporter animals at L2. n Quantification of GFP intensity measured in each epidermal nucleus (10 per animal). n = 6; error bars represent SEM; ns, not significant (Student's unpaired t-test). Primarydata for panels (b, c, and k) are provided in Additional file 14

Picture from Kim KW et al. (2016) BMC Biol "Coordinated inhibition of C/EBP by Tribbles in multiple tissues is essential for ...."

Fig. 3 NIPI-3 represses PMK-1 phosphorylation via cebp-1 and represses cebp-1 transcription. a Western blot analysis on total protein lysate fromvarious animal strains using the indicated antibodies, alpha-phospho-p38 MAPK antibody to detect a phosphorylated form of PMK-1 proteins (p-PMK-1)or alpha-actin antibody as a loading control. b Densitometric quantifications of immunoblot signals normalized to actin. n = 3; error bars represent standard error of the mean (SEM);*** P < 0.001 (one-way ANOVA with Tukey's post hoc tests). c Quantitative RT-PCR (qRT-PCR) analysis of pmk-1. Relative abundance of pmk-1mRNA normalized to actin mRNA. n = 3; error bars represent SEM; ns, not significant (one-way ANOVA with Tukey's post hoc tests). d-j Fluorescence images of cebp-1p::GFP reporter animals at L2. k qRT-PCR analysis of cebp-1 in WT, nipi-3(fr4), nipi-3(0) animals. Relative abundance ofcebp-1 mRNA normalized to actin mRNA. n = 3; error bars represent SEM; *P < 0.05; **P < 0.01; ns, not significant (one-way ANOVA with Tukey'spost hoc tests). l, m Confocal fluorescence images (z-stack) of col-154p(epidermis)::CEBP-1::GFP reporter animals at L2. n Quantification of GFP intensity measured in each epidermal nucleus (10 per animal). n = 6; error bars represent SEM; ns, not significant (Student's unpaired t-test). Primarydata for panels (b, c, and k) are provided in Additional file 14

Picture from Kim J et al. (2010) J Cell Sci "cdc-25.2, a C. elegans ortholog of cdc25, is required to promote oocyte ...."

Figure 1. Expression profile and genomic structure of cdc-25.2. (A) The expression levels of cdc-25.2 mRNA in L1, L2, L3, L4 larval, young adult (YA) and gravid adult (Ad) stages of wild-type N2 hermaphrodites, as well as in N2 adult males, in fem-1(hc17lf), fem-3(q20gf) and glp-1(q224lf) adult hermaphrodites grown at the non-permissive temperature, 25C, were measured by quantitative real-time RT-PCR. Average values from three independent experiments were normalized to that of act-1. Note the break in the y-axis. Error bars indicate the standard deviation. (B) Genomic structure of cdc-25.2, which is located on chromosome V, and consists of six exons and five introns including the very long first intron. The grey box, black boxes and carets, indicate the 5'UTR, exons, and introns, respectively. The region of the ok597 deletion and positions of primers used to detect the deletion are indicated by a dark grey rectangle and arrows, respectively.