Picture from Kim SK et al. (2000) Science "Positioning of longitudinal nerves in C. elegans by nidogen."

Figure 4. Nidogen is associated with body wall basement membranes. (A) Larva stained to reveal anti-nidogen antibodies. Intense staining is associated with the basement membrane of the body wall muscle. (B) Muscle cells in the same larva visualized by using an anti-UNC-54 myosin monoclonal antibody. The muscle cells form four sublateral rows extending the length of the body. One muscle quadrant is in the plane of view. Scale bar, 10 um.

Picture from Kim SK et al. (2000) Science "Positioning of longitudinal nerves in C. elegans by nidogen."

Figure 5. Expression of the nid-1 gene detected by using the nid-1 promoter to drive GFP expression. Embryos (A, B, C, D, and E) and larvae (F, G, H, and I). (A and B) Shortly before (A) and at the beginning (B) of embryonic elongation, expression is detected in the Cep (cephalic), IL (inner labial) and OL (outer labial) neurons (arrows). (C, D, and E) During succeeding stages of elongation until hatching, expression is detected in body wall muscle (arrowheads), the intestinal and anal depressor muscle cells (*), and the ALM neurons. (F, G, H, and I) In the larva, expression is detected in the head Ceh, IL, and OL neurons (F), the excretory cannel cell (exc) and anterior intestine (int) (G), the distal tip cells (DTC) and the HSN neurons (H), and posteriorly in the PLM neurons and the anal depressor and intestinal muscle cells (mu int and mu anal) (I).

Picture from Kim KW et al. (2018) Neuron "A Neuronal piRNA Pathway Inhibits Axon Regeneration in C.elegans."

(A) smFISH shows prde-1 mRNAs in the cytoplasm of PLM neuron labeled with Pmec-7-gfp (muIs32). Right bottom shows no probe control.

Picture from Kim KW et al. (2018) Neuron "A Neuronal piRNA Pathway Inhibits Axon Regeneration in C.elegans."

Figure 3. prde-1 mRNA Is Present in PLM, and prde-1 Inhibits Axon Regeneration Cell-Autonomously(A) smFISH shows prde-1 mRNAs in the cytoplasm of PLM neuron labeled with Pmec-7-gfp (muIs32). Right bottom shows no probe control.(B) Schematic of gfp KI into prde-1 genomic locus (ju1534). See also Figure S2A.(C) Confocal image of endogenous GFP::PRDE-1 fluorescence in the germline nuclei in the prde-1 (ju1534) gfp KI animals.(D) Schematic of depletion of GFP-tagged PRDE-1 protein using GFP-DEGRON (modeled after Wang et al., 2017).(E) PLM axon regrowth 24 hr post-axotomy. Data are represented as mean +- SEM. Student's t test. Number of animals is shown within columns. Bottom:representative images of PLM neurons 24 hr post-axotomy. Red-orange arrowhead, site of axotomy.(F) PLM axon regrowth 24 hr post-axotomy in transgenic animals expressing prde-1 driven by tissue-specific promoters for mechanosensory neurons (Pmec-4),motor neurons (Punc-25), or muscle (Pmyo-3) in a prde-1(mj207) background. Data are represented as mean +- SEM. One-way ANOVA followed by Tukey'smultiple comparison tests. Number of animals is shown within columns.

Picture from Kim J et al. (2015) PLoS Genet "The Evolutionarily Conserved LIM Homeodomain Protein LIM-4/LHX6 Specifies the ...."

Fig 6. A cis-regulatory motif is necessary and sufficient to drive expression of terminally differentiated SMB markers. (A) Shown are the predicted binding site (top) for the homeodomain of LIM-4 from a web based tool, PreMoTF (http://stormo.wustl.edu/PreMoTF) [33] and the putative cis-regulatory SMB motif (bottom) identified from promoter analysis of flp-12, odr-2, and unc-17 genes. (B) The percentage of transgenic animals expressing flp-12p::gfp reporter construct in the indicated neurons is shown. Strength of GFP expression is indicated by the number of + symbols. Wild-type nucleotides are indicated in red, mutated nucleotides in blue. At least two independent extrachromosomal lines for each construct were examined except a flp-12 promoter (-2652) construct of which number was derived from one integrated line. N is less than or equal to 50 for each. Identified cis-regulatory sequences found in other Caenorhabditis species are shown below. Analysis between -523 bp and -339 bp upstream of flp-12 promoter is shown in S10 Fig. (C) Insertion of the SMB motif into the non-SMB expressed flp-7 gene promoter induces flp-7 expression in the SMB neurons. The inserted cis-regulatory sequences identified from the flp-12 promoter analysis and the inserted site in the flp-7 promoter are indicated. GFP expression is overlapped with expression of the lim-4p3::mCherry reporter in the SMB neurons of wild-type animals. Images are derived from z-stacks of confocal microscopy images while images in the upper-left boxed regions are single focal plane confocal microscopy images. Anterior is to the left. Scale bars: 50 um. (D) The percentage of transgenic animals expressing odr-2p::gfp or unc-17p::gfp reporter construct in the indicated neurons is shown. Strength of GFP expression is indicated by the number of + symbols. Wild-type nucleotides are indicated in red, mutated nucleotides in blue. At least two independent extrachromosomal lines for each construct were examined. n50 for each. Identified cis-regulatory sequences found in other Caenorhabditis species are shown below.

Picture from Kim J et al. (2015) PLoS Genet "The Evolutionarily Conserved LIM Homeodomain Protein LIM-4/LHX6 Specifies the ...."

(A) GFP expression of oyIs35 animals carrying an integrated lim-4p::gfp reporter is overlapped with expression of the flp-12p::mCherry reporter (the SMB marker) but not with expression of the ceh-17p::dsRed reporter (the SIA marker). Images are derived from z-stacks of confocal microscopy images while images in the upper-left boxed regions are single focal plane confocal microscopy images. Anterior is to the left. Scale bar: 50 um. (B) In lim-4 mutants, expression of the lim-4p::gfp reporter is abolished in the cell bodies and processes of the SMB neurons in adult but not L1 larval stage animals. GFP expression of the lim-4p::gfp reporter is shown in wild-type(left column) or lim-4(lsk5) mutant (right column) animals at L1 larval (top) or adult stage (bottom). Note that the lim-4p::gfp reporter is not expressed in the AWB neurons of lim-4 mutants [14]. Images are derived from z-stacks of confocal microscopy images. Quantitative analysis of these phenotypes is shown in Table 1. Anterior is to the left. Scale bar: 20 um. (C) Percentage of animals of the indicated genotypes expressing stably integrated flp-12p:gfp reporter (ynIs25) is shown. Strong, weak or off expression is defined as GFP expression observed at 400x magnification in both cell bodies and processes, in only cell bodies, or not observed either in cell bodies or processes, respectively. Heat shocks were treated to L4 larval stage animals at 33C twice for 30 minutes and after 14 hours, phenotypes were analyzed. Over two independent transgenic lines were tested. n=50 for each.

Picture from Kim JS et al. (2010) Development "C. elegans STRADalpha and SAD cooperatively regulate neuronal polarity and ...."

(A) The expression of a Pstrd-1-nls::gfp reporter in the pharynx, excretory canal and the entire nervous system. (D) Sub-cellular localization of GFP-tagged, functional STRD-1 in GABAergic neurons. GFP::STRD-1 localized to the cytoplasm and the nucleus as well as along both nerve cords. Scale bars: 5 μm.

Picture from Kim HS et al. (2014) Genetics "The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell ...."

Figure 3 Expression and localization of MIG-17-Venus and Venus-MIG-18. (A and C) DIC (left), confocal (center), and merged (right) images. The enlarged images of the boxed areas in the center panels are shown on the right side. The arrowheads point to the fluorescence from the gonadal basement membrane. (A) Wild-type and mig-18(k140) young adults that express MIG-17-Venus. (B) Western blot analysis of wild-type and mig-18(k140) animals that expressed MIG-17-GFP. Worm extracts (200 mg protein/sample) were analyzed using rabbit anti-GFP (2 mg/ml, Molecular Probes). The red and blue arrowheads indicate pro- and mature forms MIG- 17-GFP, respectively. Intensities of the bands were quantified using ImageJ software. (C) Venus-MIG-18 expression in wild-type and mig-17(k174) animals. (D) Colocalization of Venus-MIG-18 and MIG-17-mCherry in the gonadal basement membrane. Confocal images for Venus (left) and mCherry (center) and DIC and confocal merged image (right). The strong dot-like signals on the upper right are of coelomocytes that take up secreted proteins. Posterior gonads are shown. Anterior to the left, dorsal to the top. Bar, 20 um.

Picture from Kim HS et al. (2014) Genetics "The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell ...."

(C) Venus-MIG-18 expression in wild-type and mig-17(k174) animals.

Picture from Kim HS et al. (2014) Genetics "The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell ...."

(D) Colocalization of Venus-MIG-18 and MIG-17-mCherry in the gonadal basement membrane. Confocal images for Venus (left) and mCherry (center) and DIC and confocal merged image (right). The strong dot-like signals on the upper right are of coelomocytes that take up secreted proteins. Posterior gonads are shown. Anterior to the left, dorsal to the top. Bar, 20 um.