Figure S3. Autophagy genes are expressed in neurons, including AIY, and active zone localization is disrupted in autophagy mutants, related to Figure 3. (A-F) Expression pattern of the autophagy genes
atg-9 (A-C) and
atg-2 (D-F) using transcriptional fusions (see Supplemental Experimental Procedures). AIY is identified with cytoplasmic mCh (B and E). (C and F) Mergeimages for (A-B) and (D-E), respectively. (G-R) Distribution of synaptic vesicles (visualized with mCh::RAB-3) and active zones (visualized with GFP::SYD1) in Zone 2 of AIY in wild type (G-I),
epg-8(
bp251) (J-L),
lgg-3(
tm1642) (M-O), and
epg-6(
bp242) (P-R) mutant animals.Each image is a maximal projection of a confocal z-stack; the asterisk denotes the location of the cell body. Scale bar in (A) for (A)-(F), 5 um, and in (G) for (G)-(R), 1 um.