Picture from Gunther CV et al. (2004) J Biol Chem "Alternative polyadenylation results in a truncated daf-4 BMP receptor that ...."

Figure 6. Changes in daf-4 transcript abundances during development. A, 1 ug of poly(A) RNA samples from developmentally synchronized populations of N2 worms were blotted and then hybridized with an exon 1-6 cDNA probe (L, larval; AD, adult; DA, dauer; PD, post-dauer). In the panel below, the signal strength (quantified using a Fuji phosphorimager) of the 2.9 kb band for each developmental stage was adjusted to equal 1 unit on the y-axis, and the intensity of the 2.0 kb band is shown as a multiple of this unit value (filled bar). B, 100-ng isolates of total RNA from developmentally synchronized N2 populations were used for real-time RT-PCR with primer sets specific for daf-4 2.0 kb mRNA (shaded bars, intron V probe) and 2.9 kb mRNA (filled bars, exon 8 probe). Template copy number for each sample was calculated by linear regression from a DNA template real-time PCR standard curve created for each probe. Changes in transcript abundance during development are depicted by calculating the ratio of PCR product produced from each primer set in the L1 stage relative to every other stage (i.e. a y-axis value of 5 would indicate that the template copy number of the tested sample is 5-fold higher than the L1 copy number). All values are the mean and S.D. of triplicate RT-PCR reactions from 2 sets (8 stages/set) of template RNAs prepared from unique populations of synchronized worms.

Picture from Johnson RW et al. (2008) Mech Dev "Positive and negative regulatory inputs restrict pax-6/vab-3 transcription to ...."

Fig. 1. Expression of a vab-3::gfp reporter transgene. (A, C, F, I) Nomarski DIC micrographs of males. In all photos, anterior is left. (D, G, J) Epi-fluorescent images of the same animals. (A) A diagram of the four post-embryonic blasts of the male hindgut and their progeny in early L2 and mid L3, and micrograph of the adult male tail. Cells of the B cell lineage (gray) generate the copulatory spicules (arrows). The Y cell progeny (black) also generate sensory structures (the post-cloacal sensilla) in adults. (B) A diagram of the genomic structure of the C. eleganspax-6 locus. Coding exons are black, with parentheses marking mab-18-specific exons. The exons coding for the DNA-binding paired domain (PD) and homeodomain (HD) are noted. Dashed arrows denote vab-3 and mab-18 transcripts. Sequences contained in the vab-3::gfp reporter transgene guEx356 are indicated as follows: solid line denotes the included vab-3 genomic sequence, gray box denotes GFP coding sequence, and dotted line denotes unc-54 3' UTR. (C and D) L2 larval vab-3 expression in unidentified neuronal cells of the head. This expression begins in embryogenesis, persists throughout the animals' lifespan, and was used as an internal control for hindgut expression experiments. (E and H) Diagrams of the early L2 (E) and mid L3 (H) male hindgut. (E, F, G) The B and Y cells have undergone one asymmetric cell division. Nuclei are labeled. vab-3 is expressed in the sensory organ precursors B.a and Y.p. (F and G) The dotted circle indicates the position of U, which lies out of the focal plane. (H, I, J) The B.a and Y.p precursors have generated eight progeny. Nuclei of B.a descendents are labeled with greek letters. vab-3 expression is robust in all B.a cell types. (I and J) The epsilon and zeta lateral pairs are out of the plane of focus (dotted circles), as are the Y.p descendents, which have differentiated and have diminished vab-3 expression.