Picture from Kemp BJ et al. (2009) Genetics "Gem-1 encodes an SLC16 monocarboxylate transporter-related protein that ...."

Figure 4. Expression of gem-1::GFP in the L1 stage gonad. (Left) DIC and (right) GFP fluorescence imaging of an L1 stage animal that carries an extrachromosomal array with a gem-1::GFP translation fusion (pEJ44). Arrow (left) indicates the Z1 gonadal precursor cell. The arrowhead in the DIC image (left) indicates an adjacent germ cell. Other bright areas in the GFP channel are due to autofluorescence.

Picture from Kemp CA et al. (2004) Dev Cell "Centrosome maturation and duplication in C. elegans require the coiled-coil ...."

SPD-2 Is a Centrosome Component. (A-J) Wild-type gonads and embryos stained for SPD-2 (red-sometimes appears yellow due to overlap with green channel), microtubules (green), and DNA (blue). Each image is a projection of multiple focal planes. SPD-2 concentrates in perinuclear foci (arrows) in the distal portion of the hermaphrodite germline (A). Such foci are absent in oocytes (Oc). Bar, 25 μm. SPD-2 also concentrates as a single focus in sperm (B). Sperm nuclei (arrows) are visible to the right of a mature oocyte. Bar, 25 μm. In one-cell embryos, SPD-2 is present at the centrosome during meiosis (C), interphase (D), prophase (E), metaphase (F), anaphase (G), and telophase (H). In (C), a meiotic spindle (arrowhead) is visible, and in (C) and (D), the sperm pronucleus and associated centrosome are indicated by arrows. SPD-2 is also present at the two-cell stage (I) and in multicellular embryos (J). Bar in (C) is 10 μm and applies to (C)-(J).(K-M) SPD-2 localizes to both PCM and centrioles. Localization of SPD-2 (K) and the centriole marker GFP-SAS-4 (L) within the same centrosome. The merged image (M) shows that a portion of SPD-2 is closely associated with the centrioles. Bar, 5 μm.

Picture from Kemp BJ et al. (2012) Curr Biol "miR-786 regulation of a fatty-acid elongase contributes to rhythmic ...."

Figure 1. mir-240/786 Is Expressed in Intestinal Cells (A) Alignment of miR-193b and miR-365a in humans with miR-240 and miR-786 in C. elegans, respectively. Dots indicate bases conserved between humans and C. elegans. (B) The mir-240 and mir-786 sequences are separated by only 100 nt on the X chromosome. The deletion, n4541, removes 1185 bp. Rescuing activity was observed with a genomic fragment that contains mir-786. (C) Schematic of C. elegans intestine with a series of 9 rings of cells from the anterior int1 to the posterior int9, modified from [17]. (D and E) Representative confocal images of the mir-240/786 promoter driving GFP expression (mir-240/786prom::gfp). Anterior is to the left. Scale bar represents 20 mm. Weak GFP expression is observed in the int1 anterior ring of cells (D). No expression is observed in the pharynx in adult worms. GFP expression is observed in the posterior int9 cells (E). Expression is also observed in the gonadal sheath cells.

Picture from Kemp BJ et al. (2012) Curr Biol "miR-786 regulation of a fatty-acid elongase contributes to rhythmic ...."

(D and E) Representative confocal images of the mir-240/786 promoter driving GFP expression (mir-240/786prom::gfp). Anterior is to the left. Scale bar represents 20 mm. Weak GFP expression is observed in the int1 anterior ring of cells (D). No expression is observed in the pharynx in adult worms. GFP expression is observed in the posterior int9 cells (E). Expression is also observed in the gonadal sheath cells.

Picture from Kemp CA et al. (2004) Dev Cell "Centrosome maturation and duplication in C. elegans require the coiled-coil ...."

Figure 3. SPD-2 Is a Centrosome Component. (A-J) Wild-type gonads and embryos stained for SPD-2 (red-sometimes appears yellow due to overlap with green channel), microtubules (green), and DNA (blue). Each image is a projection of multiple focal planes. SPD-2 concentrates in perinuclear foci (arrows) in the distal portion of the hermaphrodite germline (A). Such foci are absent in oocytes (Oc). Bar, 25 μm. SPD-2 also concentrates as a single focus in sperm (B). Sperm nuclei (arrows) are visible to the right of a mature oocyte. Bar, 25 μm. In one-cell embryos, SPD-2 is present at the centrosome during meiosis (C), interphase (D), prophase (E), metaphase (F), anaphase (G), and telophase (H). In (C), a meiotic spindle (arrowhead) is visible, and in (C) and (D), the sperm pronucleus and associated centrosome are indicated by arrows. SPD-2 is also present at the two-cell stage (I) and in multicellular embryos (J). Bar in (C) is 10 μm and applies to (C)-(J).(K-M) SPD-2 localizes to both PCM and centrioles. Localization of SPD-2 (K) and the centriole marker GFP-SAS-4 (L) within the same centrosome. The merged image (M) shows that a portion of SPD-2 is closely associated with the centrioles. Bar, 5 μm.(N-T) Time-lapse confocal images of a strain coexpressing GFP-SPD-2 and GFP-Histone. GFP-SPD-2, indicated by arrows, is detected at centrosomes which are initially found in close association with the male pronucleus (N), and later with the two conjoined pronuclei (O). During each mitosis, GFP-SPD-2 localizes to the spindle poles (P-T). A polar body (pb) labeled by the GFP-Histone marker is visible in (O)-(S). Bar, 10 μm.

Picture from Kemp CA et al. (2004) Dev Cell "Centrosome maturation and duplication in C. elegans require the coiled-coil ...."

(N-T) Time-lapse confocal images of a strain coexpressing GFP-SPD-2 and GFP-Histone. GFP-SPD-2, indicated by arrows, is detected at centrosomes which are initially found in close association with the male pronucleus (N), and later with the two conjoined pronuclei (O). During each mitosis, GFP-SPD-2 localizes to the spindle poles (P-T). A polar body (pb) labeled by the GFP-Histone marker is visible in (O)-(S). Bar, 10 um.

Picture from Bandyopadhyay J et al. (2002) Mol Biol Cell "Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is ...."

CNB-1::GFP expresses in spermatheca (C; arrowhead) and intestine (C; arrow). Nerve cord expression can also be observed in this image.

Picture from Geisler F et al. (2016) Mol Biol Cell "A novel function for the MAP kinase SMA-5 in intestinal tube ...."

The pancytoplasmic localization of SMA-5::EGFP inthe intestine can be appreciated in B' and B'' (nuclei devoid of fluorescence marked by asterisks).

Picture from Inoue T et al. (2002) Mech Dev "Gene expression markers for Caenorhabditis elegans vulval cells."

Side-by-side comparison of markers disregarding the temporal aspect,demonstrating that six different cell types can be distinguished based on the expression pattern.

Picture from Alexander M et al. (2009) Development "An UNC-40 pathway directs postsynaptic membrane extension in Caenorhabditis ...."

(G) Functional UNC-97::GFP (Hobert et al., 1999) fusion protein can be seen in the muscle arms (red arrows). Scale bar 50 μm.