Picture from Keating CD et al. (2003) Curr Biol "Whole-genome analysis of 60 G protein-coupled receptors in Caenorhabditis ...."

Figure 3. The Role of C10C6.2 in Locomotion. (A and B) A comparison of wave form in (A) wild-type and (B) animals treated with RNAi for C10C6.2. Note the flattened wave form in the RNAi-treated animal.(C) The expression pattern of C10C6.2 was determined by using a C10C6.2::DsRed2 reporter construct containing 1965 nucleotides of the 5' flanking region and 658 nucleotides of the C10C6.2 coding sequence in frame with DsRed2.(D) This construct was coinjected with pCelica-P-GFP for pan-neuronal expression of a Ric-19-GFP fusion protein [26].(C) and (D) are images of the same animal that indicate that C10C6.2 is expressed in all of the neurons of the ventral nerve cord. The position of the nerve cord is indicated by the arrows.

Picture from Keating CD et al. (2003) Curr Biol "Whole-genome analysis of 60 G protein-coupled receptors in Caenorhabditis ...."

(C) The expression pattern of C10C6.2 was determined by using a C10C6.2::DsRed2 reporter construct containing 1965 nucleotides of the 5' flanking region and 658 nucleotides of the C10C6.2 coding sequence in frame with DsRed2. (D) This construct was coinjected with pCelica-P-GFP for pan-neuronal expression of a Ric-19-GFP fusion protein [26].(C) and (D) are images of the same animal that indicate that C10C6.2 is expressed in all of the neurons of the ventral nerve cord. The position of the nerve cord is indicated by the arrows.

Picture from Ginzburg VE et al. (2002) Development "Semaphorin 1a and semaphorin 1b are required for correct epidermal cell ...."

smp-2 expression patterns visualized with gfp transcriptional reporters during embryogenesis. DIC (left) and GFP (right) images of the same embryo. (G,H) Initial embryonic expression of smp-2::gfp is observed at the twofold stage in pharyngeal (arrows) and intestinal (arrowheads) cells. These cells were identified using SIMI BioCell CD ROMTM (Schnabel et al., 1997).

Picture from Ginzburg VE et al. (2002) Development "Semaphorin 1a and semaphorin 1b are required for correct epidermal cell ...."

Figure 8. smp-1 and smp-2 expression patterns visualized with gfp transcriptional reporters during embryogenesis. DIC (left) and GFP (right) images of the same embryo. (A,B) The expression pattern of smp-1::gfp can be initially observed at approximately 150 minutes in four cells of the E lineage and later at about 250 minutes of development spread to eight cell of the same lineage. (C,D) At the time of ventral enclosure, the leading edge epidermal cells and the muscle lineage start to express smp-1::gfp. (E,F) In the comma stage, muscle precursors and other cells in the head express smp-1::gfp. (G,H) Initial embryonic expression of smp-2::gfp is observed at the twofold stage in pharyngeal (arrows) and intestinal (arrowheads) cells. These cells were identified using SIMI BioCell CD ROMTM (Schnabel et al., 1997).

Picture from Kim YI et al. (2004) J Mol Biol "Transcriptional regulation and life-span modulation of cytosolic aconitase ...."

Figure 4. Expression profiles of ferritins. A-H, ftn-1::gfp expression worms observed under DIC (A, C, E and G) and fluorescent microscope (B, D, F and H). Transgenic animals show GFP expression in the intestine of wild-type (A and C) and ftn-2(ok404) (E and G) on normal NGM plate. I-L, ftn-2::gfp expression worms observed under DIC (I and K) and fluorescent microscope (J and L). Transgenic animals show GFP expression in pharyngeal muscle and hypodermis of wild-type on normal NGM plate. ftn-1::gfp expression worms on FAC containing plates show much stronger GFP expression and bright dotted pattern (compare AB with EF and CD with GH). ftn-2::gfp expression worms on FAC plate were not much induced (compare IJ with KL). ftn-1::gfp expression level was strongly increased in ftn-2(ok404) (compare EF with GH). ftn-2::gfp expression level was not much induced in FAC (compare IJ with KL). The bar represents 100 um.

Picture from Nikonorova IA et al. (2022) Curr Biol "Isolation, profiling, and tracking of extracellular vesicle cargo in ...."

Figure S5: Different neuronal types carry distinct EV cargo; a single neuronal cilium may shed multiple cargoes, related to Figure 5.
(A) Sagittal image through the C. elegans embryo shows nuclear localization of MCM- 3::mScarlet in the actively dividing cells. (B) MCM-3 presence in cilia of the CEM and IL2 neurons and environmental EVs. (C-D) MCM-3::mScarlet presence in EV-releasing ciliated neurons in the head - IL2s and CEMs (C), and in the tail - RnBs and HOB (D). The MCM-3::mScarlet reporter also shows weak presence in the rectal gland. This identification is based on its similarity to an image in the WormAtlas describing anatomical structures of male proctodeum [S3]. (E) Ortholog of human CD-9 (TSP-6) is expressed in the amphid ciliated neurons, but not in the PKD-2-expressing EV-releasing neurons. (F) TSP-6::mScarlet EV release from amphid channel cilia.
PKD-2::GFP served as a marker of the CEM, RnB, and HOB neurons. Cilia of the RnB neurons are indicated with numbers.