Figure 1. TMC-1 is expressed in ALA, functions in ALA-dependent inhibition of egg-laying, but does not appear to function in response to harsh touch in ALA: (A) A transcriptional reporter of
tmc-1 (ljEx1221[Ptmc-1(4kb)::mKate2]) is expressed in the ALA neuron (marked by ljEx463 [Pida- 1::GFP]). Scale bar 10μm. (B) Egg-laying rate in response to an anterior harsh touch. Each data point presents one individual animal, eggs were counted after 3 hours and normalised to one hour. A One-way ANOVA was statistically significant (p=0.0023), a post-hoc test (Bonferroni) revealed a significant difference between the response to anterior touch in
tmc-1 mutants and wild-type. (C) Wild-type (blue) and
tmc-1 mutant (brown) ALA calcium transients in response to a 1 second anterior harsh touch stimulus (arrow; 40psi). Mean and SEM are shown. (D) Maximum ratio change of GCaMP3 fluorescence shows no statistically significant difference between
tmc-1 mutants and the wild-type. A Mann-Whitney U test was non-significant. (E) Time to maximum after delivery of stimulus. Time measured from stimulus to the maximum of GCaMP fluorescence does not show statistically significant difference between
tmc-1 mutants and the wild-type. A Mann-Whitney U test was non-significant. Error bars for each panel represent median ± interquartile range (IQR) unless otherwise stated. Every data point presents an individual animal. The numbers above the graphs present p-values for the respective statistical test and number underneath the graph indicates number of total animals used for each condition.