Picture from Kato Y et al. (2002) Biochem J "abf-1 and abf-2, ASABF-type antimicrobial peptide genes in Caenorhabditis ...."

ABF-2-GFP was mainly detected in the pharyngeal tissue.

Picture from Kato Y et al. (2002) Biochem J "abf-1 and abf-2, ASABF-type antimicrobial peptide genes in Caenorhabditis ...."

Northern-blot analysis. Total RNA (20 μg) was loaded. As a control for loading calibration, the C. elegans homologue of eif was used. The abf-1 transcripts were detected as five bands. The short SL-less and long SL-trans-spliced transcripts of abf-1 could be the 0.3 and 0.5 kb bands. In contrast, the abf-2 transcript was detected as a single band (0.4 kb, identical with the short SL-less form). The accumulation of both abf-1 and abf-2 transcripts was almost identical in adults of N2 and glp-4(bn2), and the eggs.

Picture from Kato Y et al. (2002) Biochem J "abf-1 and abf-2, ASABF-type antimicrobial peptide genes in Caenorhabditis ...."

ABF-1-GFP was observed in the pharyngeal lumen.

Picture from Kato Y et al. (2002) Biochem J "abf-1 and abf-2, ASABF-type antimicrobial peptide genes in Caenorhabditis ...."

Figure 5. Expression of abf genes. Expression of abf-gfp fusion genes in transgenic animals. ABF-1-GFP (construct 1 in Figure 3, bottom right-hand panel) was observed in the pharyngeal lumen. ABF-2-GFP (construct 2 in Figure 3, bottom right-hand panel) was mainly detected in the pharyngeal tissue.

Picture from Kato Y et al. (2002) Biochem J "abf-1 and abf-2, ASABF-type antimicrobial peptide genes in Caenorhabditis ...."

Figure 4. Immunofluorescent detection of ABF-2. (A and B) Animals were immunostained with an anti-[ABF-2 (Cys60-Ala89)] antibody. ABF-2 was detected only in the pharyngeal marginal zone. (C and D) Pre-immune IgG instead of the anti-(ABF-2) antibody was used for immunostaining. (E and F) Secondary antibody alone without the anti-(ABF-2) antibody. (A, C and E) Images taken using Nomarski differential interference contrast microscopy. (B, D and F) Fluorescent images.

Picture from Inoue T et al. (2004) Proc Natl Acad Sci U S A "Type II platelet-activating factor-acetylhydrolase is essential for ...."

(I and J) paf-2::GFP expression in embryos (lateral view, dorsal is up). Nomarski micrographs (I) and corresponding paf-2::GFP expression (J). paf-2 reporter gene constructs are expressed in epidermal cells (J, arrowheads) and intestinal cells (J, arrow) at the beginning of elongation. Anterior is to the left. (Bar, 10 um.)

Picture from Solari F et al. (1999) Development "The Caenorhabditis elegans genes egl-27 and egr-1 are similar to MTA1, a member of ...."

(J,K) egl-27::gfp expression in 1.5-fold embryo (J) and L3 hermaphrodite (K). All somatic cells appear to express the reporter gene.

Picture from Zhang D et al. (2012) J Cell Biol "RAB-6.2 and the retromer regulate glutamate receptor recycling through a ...."

Figure 2. RAB-6.2 is broadly expressed in multiple tissues. (A-I) Fluorescence from Prab-6.2::GFP in body wall muscles (A), pharyngeal muscle and head neurons (B), hypodermal cells (C), the seam cells (D), intestinal epithelia (E), coelomocytes (F), vulval epithelia (G), the distal tip cells (H), and the vulval muscles and ventral cord dendrites (I). (J-J'') Fluorescence from Prab-6.2::GFP (J) and Pglr-1::mRFP (J') with images merged (J''). Coexpressing neurons are outlined. Bars, 5 um.

Picture from Zhu Z et al. (2016) Dev Cell "Functional Coordination of WAVE and WASP in C.elegans Neuroblast ...."

(J-L) Fluorescence images (J) and quantification(K and L) of ARX-2 (GFP knockin). (J) Upper,merged image; middle, plasma membrane andhistone; lower, ARX-2. Arrows, leading edges;asterisks, ARX-2::GFP from an adjacent apoptoticcell. Box plots show the quantification of ARX2::GFP enrichment

Picture from Nehrke K et al. (2002) J Biol Chem "The NHX family of Na+-H+ exchangers in Caenorhabditis elegans."

I and J, nhx-3:: GFP expression in hypodermal cells (hyp).