- Picture from Karabinos A et al. (2003) J Mol Biol "In vivo and in vitro evidence that the four essential intermediate filament (IF) ...."
Figure 4. Colocalization of B1 and MH4 antigens in C. elegans embryo and larva. The left half of the Figure gives rabbit anti-B1 specific immunofluorescence of embryos (A, C and E) and the larva (G) that were double-stained with the murine monoclonal antibody MH4 that detects proteins A1 to A3 (right half of the Figure). The arrowheads highlight coexpression/co-organization of the B1 and the MH4 antigens in the dorsal and ventral hypodermis (except for the seam) of the comma (A and B), 1.5-fold (C and D) and 3-fold (E and F) stage embryos and the larva (G and H).
- Picture from Karabinos A et al. (2002) Mech Dev "Expression profiles of the essential intermediate filament (IF) protein A2 and ...."
Fig. 2. Tissue specific expression of the A2-promoter/gfp construct in larva. The 1509 nt long 5'-upstream region of the A2 gene (Fig. 1B) was used in the promoter/gfp reporter. Only F2 generation animals were photographed. Due to the toxicity of the transgene it was not possible to propagate extrachromosomal arrays any further. Nomarski phase contrast monitoring (A) was used to facilitate the identification of cells and tissues. Expression of GFP in larva was detected in the main body hypodermis excluding the seam cells marked by arrows. These appear as dark gaps on the green GFP fluorescence of the hypodermal syncytium (B). Note the weak fluorescence in the ventral nerve cord and the intense GFP fluorescence of unidentified structures in the tail. Bar 25 um.
- Picture from Karabinos A et al. (2003) J Mol Biol "In vivo and in vitro evidence that the four essential intermediate filament (IF) ...."
Figure 3. Tissue-specific expression of the B1a and A4-promoter/gfp constructs in larva and adult. Expression of the B1a-promoter driven GFP in the larva was detected in the main body hypodermis (excluding the seam cells), the pharynx, the excretory cells and the rectum (A). This expression pattern and the additional expression in the vulva were seen in the late larva/juvenile (marked by arrows in B-D), while no signals were detected in the adult hypodermis (marked by arrowheads in C and D). Expression of the A4-promoter driven GFP in larva was detected in the pharyngeal-intestinal valve and the rectum (E). This and the additional staining in the H-shaped excretory cells (F and H) and some other unidentified interior cells (G) were seen in the adult. The A4-promoter/gfp construct was upregulated strongly in the newly formed dauer larva (I and its higher magnification in J). Scale bars represent 50 um for A-H and 25 um for I and J.
- Picture from Karabinos A et al. (2003) J Mol Biol "In vivo and in vitro evidence that the four essential intermediate filament (IF) ...."
Figure 2. Tissue-specific expression of the A1a-promoter/gene/gfp construct in larva and adult. The 1946 nt 5'-upstream region plus the entire A1 gene (see Figure 1B, and Materials and Methods) were used in the A1a-promoter/gene/gfp reporter. Nomarski phase contrast monitoring (B, D, F and H) was used to facilitate the identification of cells and tissues. In the larva, expression of the A1a-GFP was detected in the filament bundles of the pharyngeal marginal cells, the rectum and the vulva (the latter marked by the arrow in A). C, E and G Higher magnifications of the pharynx, vulva and rectum of the larva given in A. In addition, strong A1a-GFP-fluorescent filament bundles were present in the uterus of the adult (I). Scale bars represent 25 um.
- Picture from Karabinos A et al. (2001) Proc Natl Acad Sci U S A "Essential roles for four cytoplasmic intermediate filament proteins in ...."
Figure 4. Expression of A1-, A3-, and B1-promoter/gfp constructs and the IF protein B1. Expression of the A1 promoter/gfp reporter in cells associated with amphid sensory neurons (A), the pharyngeal-intestinal valve (C), the rectum, and some neurons of the tail (E). Similar structures were immunostained with antibody MH4 which, however, also decorates the hypodermis (B, D, F, and J). The A3 promoter/gfp reporter is primarily expressed in the embryonal and larval hypodermis (G). The B1 promoter/gfp is expressed in the rectum and some unidentified neurons in mixed-stage animals (H). Whole-mount worms were double-labeled with affinity-purified rabbit antibody specific for IF protein B1 (I) and with antibody MH4 (J). [Scale bars: 50 um (A, C, E, and H) and 25 um (B, D, F, G, I, and J).]