Picture from Kao G et al. (2007) Cell "ASNA-1 positively regulates insulin secretion in C. elegans and mammalian ...."

Figure 3. ASNA1 Is Expressed in Cells that Produce Insulin or Insulin-Like Proteins in Humans and C. elegans. (A and C). Micrographs of worms of the genotype svIs56 viewed with Nomarski DIC optics. svIs56 is a transgene array containing full-length asna-1::GFP under the control of the asna-1 promoter. Arrows in (C) indicate the position of the intestine. (B and D). Micrographs of the same worms viewed with fluorescence optics. Scale bars are 25 μm. The amphid neurons ASI and ASK are indicated. Arrow in (D) indicates cells expressing the fusion protein. (E-H) Micrographs of human pancreatic tissue viewed with fluorescence optics (E and F) or light optics (G and H) at 40 magnification. (F and H) Tissue stained with an antibody recognizing human insulin. (E and G) Tissue stained with an antibody recognizing human ASNA1. The arrows indicate cells expressing insulin and ASNA1. The arrowheads indicate other pancreatic islet cells that express neither insulin nor ASNA1. (G) and (H) show consecutive thin sections; the regions stained brown (indicated by arrows) are those expressing insulin and ASNA1, respectively.

Picture from Kao G et al. (2007) Cell "ASNA-1 positively regulates insulin secretion in C. elegans and mammalian ...."

(A and C). Micrographs of worms of the genotype svIs56 viewed with Nomarski DIC optics. svIs56 is a transgene array containing full-length asna-1::GFP under the control of the asna-1 promoter. Arrows in (C) indicate the position of the intestine. (B and D). Micrographs of the same worms viewed with fluorescence optics. Scale bars are 25 μm. The amphid neurons ASI and ASK are indicated. Arrow in (D) indicates cells expressing the fusion protein.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

A) A confocal image ofanimals expressing a functional UBR-1::GFP transgene from its endogenous promoter(green), and the Pnmr-1-RFP reporter (red).