Picture from Margalit A et al. (2007) J Cell Biol "Barrier to autointegration factor blocks premature cell fusion and maintains ...."

GFP-BAF-1 protein expression. (A) Schematic view of the baf-1- gfp construct used for generating transgenic strains. It includes the baf-1 promoter driving the gfp gene fused to the baf-1 ORF followed by the baf-1 3' untranslated region. (B-D) A stronger GFP-BAF-1 signal was detected at the nuclear periphery, whereas weaker signals were detected in the nucleoplasm and cytoplasm. GFP-BAF-1 was detected in the gonad (arrowhead in B) in young embryos (arrows in B) and in late embryos (not depicted), in L1 larvae (C), and in adult worms (arrow in D points toward the vulva). The image in B was obtained with a confocal microscope, whereas images in C-D were viewed with an Axioplan II microscope (Carl Zeiss MicroImaging, Inc.). Bars, 10 um.

Picture from Fujiwara M et al. (2015) Genetics "The Importance of cGMP Signaling in Sensory Cilia for Body Size Regulation in ...."

Figure 5 Expression analyses of gcy-12. Schematic of gfp expression constructs and the projections of confocal images are shown. An LSM510 confocal microscope (Carl Zeiss) was used to scan GFP and mRFP fluorescence. The nose tip is to the left (A and B). (A) The expression patterns of genomic gcy-12::gfp in thehead regions of wild-type and che-2(e1033) animals at the L4 stage. Scale bar, 20 um. (B) The expression patterns of odr-1p::gcy-12::gfp in the head region of a wild-type animal at the adult stage. The arrow indicates the dendrite tip and the dashed circle indicates cell bodies of AWC and AWB neurons. Scale bar, 20 um.(C) The localization patterns of GCY-12::GFP in the sensory cilia of PHA/PHB neurons in the tail. The cilia (brackets) were visualized with srb-6p::mRFP. Scale bar, 5 um.

Picture from Margalit A et al. (2007) J Cell Biol "Barrier to autointegration factor blocks premature cell fusion and maintains ...."

Figure 1. GFP-BAF-1 protein expression. (A) Schematic view of the baf-1- gfp construct used for generating transgenic strains. It includes the baf-1 promoter driving the gfp gene fused to the baf-1 ORF followed by the baf-1 3' untranslated region. (B-D) A stronger GFP-BAF-1 signal was detected at the nuclear periphery, whereas weaker signals were detected in the nucleoplasm and cytoplasm. GFP-BAF-1 was detected in the gonad (arrowhead in B) in young embryos (arrows in B) and in late embryos (not depicted), in L1 larvae (C), and in adult worms (arrow in D points toward the vulva). (E and F) DIC, left; GFP fluorescence, right. Although GFP-BAF-1 was localized normally at the nuclear periphery of control embryos (L4440; E), it was mislocalized in lmn-1(RNAi) embryos (arrowheads in F), where the GFP-BAF-1 was also found associated with anaphase chromatin bridges (arrow in F). The image in B was obtained with a confocal microscope, whereas images in C-F were viewed with an Axioplan II microscope (Carl Zeiss MicroImaging, Inc.). Bars, 10 um.