Picture from Jospin M et al. (2007) Curr Biol "UNC-80 and the NCA ion channels contribute to endocytosis defects in ...."

(B) unc-80 is strongly expressed in neurons. An adult hermaphrodite expressing Punc-80:GFP is shown, ventral side is up. Strong expression is observed in the nervous system and in the vulval muscle. Below are magnified images showing expression in the anterior excretory canal just posterior of the head, the vulval muscle, distal tip cell of the gonad, and the anal depressor muscle in the tail, lateral views. Scale bars represent 10 um.

Picture from Jospin M et al. (2009) PLoS Biol "A neuronal acetylcholine receptor regulates the balance of muscle excitation ...."

Figure 3. acr-2 is expressed and functions in the cholinergic ventral cord motor neurons. (A) Expression pattern from a 3.5-kb acr-2 promoter-driven Pacr-2-GFP transgene (juIs14) in an L1 (upper image) and an L4 larva. Middle image is a side view, and the bottom image is a ventral view of an L4 larva. The number and positions of the cells and the sides of the commissures indicate that they are embryonically born DA and DB and postembryonically born VA and VB neurons. Expression is also seen in DVC in the tail (arrow, middle panel). Scale bars indicate 10 µm. (B). The top image shows nonoverlapping expression of Pacr-2-GFP (juIs14) (green) and Pttr-39-mcherry (juIs223) (red) marking the DD and VD GABAergic neurons. The bottom images show nonoverlapping expression of the 1.8-kb promoter-driven Pacr-2-mcherry transgene (juEx2045) (red) and those of Pglr-1-GFP (nuIs1) (green) or Pnmr-1-GFP (akIs3) (green), which label the command interneurons in the head (left panels) and tail (right panels) ganglia. Transcriptional activity of the acr-2 1.8-kb promoter is not seen in any head neurons. Scale bars indicate 10 µm. (C) Quantification of cell-type-specific transgenic rescue of the convulsion defects in acr-2(n2420gf) animals. Pacr-2::acr-2 contains the full-length genomic coding sequence driven by the 3.5-kb-long promoter. Pacr-2::acr-2(mini) contains cDNA that replaced genomic DNA from exon 2, driven by the 1.8-kb promoter. Punc-25::acr-2 contains the full-length genomic coding sequences driven by the 1.4-kb unc-25 promoter. n = 10, 10, 5, 10, and 8 for N2, acr-2(n2420gf), acr-2(n2420gf);Pacr-2-acr-2(oxEx707), acr-2(n2420gf); Pacr-2-acr-2(mini) (juEx2336), and acr-2(n2420gf);Punc-25-acr-2(juEx32), respectively.

Picture from Jospin M et al. (2007) Curr Biol "UNC-80 and the NCA ion channels contribute to endocytosis defects in ...."

Figure 1. unc-80 Encodes a Large, Conserved Neuronal Protein. (A) unc-80 encodes a protein predicted to be 3184 amino acids in length (splice form F25C8.3a). Dark regions indicate strong conservation with homologs in other species. The percent identity between C. elegans and homologous proteins in Drosophila (CG18437-PA), rat (XP_001053529), and canine (XP_545626) for each of these regions is shown below. Partial sequences for mouse (XP_898856) and human (C2orf21/EAW70465) also exist. A Heat domain was predicted by SMART, with a score of 9 e-06, but not by other domain search programs including Interpro and Motif scan.(B) unc-80 is strongly expressed in neurons. An adult hermaphrodite expressing Punc-80:GFP is shown, ventral side is up. Strong expression is observed in the nervous system and in the vulval muscle. Below are magnified images showing expression in the anterior excretory canal just posterior of the head, the vulval muscle, distal tip cell of the gonad, and the anal depressor muscle in the tail, lateral views. Scale bars represent 10 um.

Picture from Jospin M et al. (2009) PLoS Biol "A neuronal acetylcholine receptor regulates the balance of muscle excitation ...."

acr-2 is expressed and functions in the cholinergic ventral cord motor neurons. (A) Expression pattern from a 3.5-kb acr-2 promoter-driven Pacr-2-GFP transgene (juIs14) in an L1 (upper image) and an L4 larva. Middle image is a side view, and the bottom image is a ventral view of an L4 larva. The number and positions of the cells and the sides of the commissures indicate that they are embryonically born DA and DB and postembryonically born VA and VB neurons. Expression is also seen in DVC in the tail (arrow, middle panel). Scale bars indicate 10 um. (B). The top image shows nonoverlapping expression of Pacr-2-GFP (juIs14) (green) and Pttr-39-mcherry (juIs223) (red) marking the DD and VD GABAergic neurons. The bottom images show nonoverlapping expression of the 1.8-kb promoter-driven Pacr-2-mcherry transgene (juEx2045) (red) and those of Pglr-1-GFP (nuIs1) (green) or Pnmr-1-GFP (akIs3) (green), which label the command interneurons in the head (left panels) and tail (right panels) ganglia. Transcriptional activity of the acr-2 1.8-kb promoter is not seen in any head neurons. Scale bars indicate 10 um.

Picture from Goh KY et al. (2018) Biochim Biophys Acta "A large transcribed enhancer region regulates C. elegans bed-3 and the ...."

(A) bed-3 is expressed in the M lineage including M descendants and SM descendants.

Picture from Yang Q et al. (2017) Dev Cell "The Invading Anchor Cell Induces Lateral Membrane Constriction during Vulval ...."

(M-P) Localization of the endogenous GFP::TOCA-1 reporter zh110 in wild-type (M) and unc-6(ev400) mutants (O and P) before (M and O) and after (N and P)lumen formation. (M') and (O') show yz projections of the lateral VulF membranes. The inset in (O) shows the displaced AC in the unc-6(ev400) mutant in a differentfocal plane.

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).

Picture from Hashimshony T et al. (2015) Nature "Spatiotemporal transcriptomics reveals the evolutionary history of the ...."

2. Hashimshony, T., Feder, M., Levin, M., Hall, B. K. & Yanai, I. Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer. Nature 519, 219-22 (2014).