Figure 2. WAH-1 localizes to mitochondria and can be released by EGL-1. The Nomarski, GFP, and Mitotracker Red images and the merged image of GFP and Mitotracker Red (from left to right) of an early C. elegans embryo from the following strains are shown: N2 animal transgenic for Pwah-1
wah-1::gfp(A);
ced-1(
e1735);
egl-1(
n3082) animal transgenic for both Pwah-1
wah-1::gfpand Phsp
egl-1 constructs without heat-shock treatment (B) or after heat-shock treatment at 33 C for 1 hour (C) (16);
ced-1(
e1735);
ced-3(
n2433);
egl-1(
n3082) animal transgenic for both Pwah-1
wah-1::gfp and Phsp
egl-1 after heat-shock treatment at 33 C for 1 hour (D and E) (16). WAH-1::GFP was not released from mitochondria in the embryo shown in (D) but was partially released in the embryo shown in (E).