Picture from Garzanelli, John et al. (2020) MicroPubl Biol

Percentage of embryos that failed to hatch after feeding RNAi knockdown in wildtype (A) or unc-33(e204) homozygous animals (B): Box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. Horizontal bars indicate the medians, asterisks indicate the means, and polygons represent density estimates of data and extend to extreme values. From left to right in the plot, n=24, 23, 24, 23, 23, 24, where each n is the percentage that failed to hatch from a single hermaphrodite. Standard deviations from left to right are 4.8, 14.8, 4.3, 19.2, 18.4, 30.9.

Picture from Dixon SJ et al. (2005) Development "Muscle arm development in Caenorhabditis elegans."

Figure 1. The muscle arms of C. elegans. (A) An illustration of the BWMs (red) of the left side of C. elegans. Each BWM quadrant is organized into two rows. (B) A cross-section of A adapted, with permission, from White et al. (White et al., 1986). Each muscle of the two dorsal BWM quadrants and each muscle of the two ventral quadrants (red) extends arms to the nearest nerve cord (dark green) in either the DHR or the VHR, respectively. Only the muscle arms of the ventral left quadrant are indicated by green arrows. The LHR is indicated. (C) A transmission electron micrograph by John White (with permission) of the boxed region in B. BWMs are shaded pink. (D) The dorsal right (top) and dorsal left (bottom) BWMs that express MB::YFP from the trIs30 integrated transgene in a young adult. The Cpppaaa BWM is indicated (yellow arrow), along with four muscle arms (green arrows). (E) The ventral left (top) and ventral right (bottom) BWMs. The red asterisk indicates the position of the vulva. (F,G) Tracings of the dorsal and ventral BWMs, respectively. The identities of the distal muscles that express Mb::YFP at high levels are bright yellow, and those that express Mb::YFP at lower levels are light yellow. The head BWMs are shaded in dark grey, while the neck BWMs are shaded in light grey. The black X' in (G) represents the vulval muscles, which do not express Mb::YFP from trIs30 transgene. Anterior is to the left in all panels except in B and C. Scale bars: 50 μm. DHR, dorsal hypodermal ridge; LHR, lateral hypodermal ridge; VHR, ventral hypodermal ridge.

Picture from Jedrusik MA et al. (2002) J Cell Sci "A novel linker histone-like protein is associated with cytoplasmic filaments in ...."

Fig. 7. Embryonic expression and subnuclear localization of H1.X. (A,B) show H1.X::GFP fluorescence detection in a few cells in the periphery of a >100-cell stage embryos. In (A), a fixed specimen shows a shallow fluorescence of the nucleoplasm and bright fluorescence of the nucleoli, whereas in (B), a live observation shows a shallow fluorescence in the cytoplasm and a bright fluorescence of the nucleoplasm. The brightest spots in the nucleoplasm correspond to the nucleoli. Bars, 20 μm.

Picture from Chen F et al. (2000) Science "Translocation of C. elegans CED-4 to nuclear membranes during programmed cell ...."

(A) CED-9 expression in a WT embryo of ~30 to 50 cells. (B) Mitotracker Red localization in the same embryo as in (A). (C) Merged image of (A) and (B).

Picture from van Wolfswinkel JC et al. (2009) Cell "CDE-1 affects chromosome segregation through uridylation of CSR-1-bound ...."

ISH of whole mount and extruded gonads with a probe antisense to cde-1. A sense probe was used as a control.

Picture from Zhang S et al. (2004) Curr Biol "MEC-2 is recruited to the putative mechanosensory complex in C. elegans touch ...."

(A) MEC-2 and UNC-24::GFP puncta colocalize in a PLM process.

Picture from Kritikou EA et al. (2006) Genes Dev "C. elegans GLA-3 is a novel component of the MAP kinase MPK-1 signaling pathway ...."

(A) A Northern blot of total RNA isolated from synchronized wild-type animals was probed with a gla-3 cDNA as described in Materials and Methods. Beta-Tubulin was used as a loading control.

Picture from Millonigg S et al. (2014) PLoS Genet "GLD-4-mediated translational activation regulates the size of the ...."

(A) GLD-4 expression is equal across the distal germ line. GLD-2 intensities increase from low-to-high in a distal-to-proximal manner. Extruded gonads of indicated genotype stained with DAPI, a-GLD-2, a-GLD-4, and a-GLH-2 as a positive tissue penetration control (not shown). Asterisk, distal tip; arrowhead, mitosis-to-meiosis boundary.

Picture from Xu XZS et al. (2003) Cell "A C. elegans sperm TRP protein required for sperm-egg interactions during ...."

Figure 3. The TRP-3 Protein Is Highly Enriched in Sperm. (A-H) An affinity-purified antibody against TRP-3 specifically stains sperm in wild-type hermaphrodites. Scale bar = 10 μm.(A) An immunofluorescence image of anti-TRP3 staining of a wild-type hermaphrodite.(B) The same animal in (A) stained with DAPI.(C) A superimposed image of (A) and (B).(D) A DIC image of the same animal in (A-C).(E-H) The TRP-3 antibody is specific.(E) The TRP-3 antibody does not detect a signal in trp-3(sy693) mutants.(F) A DAPI image of the same animal shown in (E).(G) A superimposed image of (E) and (F).(H) A DIC image of the same animal shown in (E-G).

Picture from Chitturi J et al. (2018) PLoS Genet "The UBR-1 ubiquitin ligase regulates glutamate metabolism to generate ...."

A) A confocal image ofanimals expressing a functional UBR-1::GFP transgene from its endogenous promoter(green), and the Pnmr-1-RFP reporter (red).