Figure 7. Identity of the neurons expressing cegat-1 gene in C. elegans. Expression of the cegat-1 gene in C. elegans was determined by a GFP reporter fusion construct. The construct was designed such that the expression of GFP was driven by the cegat-1 gene-specific promoter. This construct was then used to generate GFP-expressing transgenic worms. The top panel identifies the GFP-positive DD and VD neurons in the ventral cord (10x), the middle panel identifies the GFP-positive neurons in the tail region (20x), and the bottom panel identifies the GFP-positive neurons in the head region (40x).
Expression pattern of C01H6.9 (haspin; G) as determined by promoter-GFP reporter constructs. (G) C01H6.9 expression is shown in one of the phasmid neurons, PHB.
Expression of MBOA-7::GFP in an early embryo (D and E) and a second-stage larva (F and G). Nomarski micrographs (D and F) and corresponding MBOA-7::GFP expression (E and G). MBOA-7::GFP was ubiquitously expressed throughout development. Asterisks indicate the anterior of the larva (F and G). Bar, 10 um (D and E) and 200 um (F and G).
(G and H) MNR-1 was only expressed in the hypodermis (H) and showed no obvious subcellular localization (G). Arrowhead in (H) indicates fluorescent labeling of the hypodermal cells.