Picture from Steger KA et al. (2004) Genetics "The GAR-3 muscarinic receptor cooperates with calcium signals to regulate ...."

Figure 2. gar-3 encodes a muscarinic acetylcholine receptor expressed in neurons and in the pharyngeal muscle. (A) Alignment of the N-terminal portion of the GAR-3 amino acid sequence with human muscarinic receptors (the GAR-3 sequence data are available in the GenBank databases under accession no. AF139093). The vu78 point mutation is indicated by an arrowhead. The lg1201 deletion is underlined. The deletion also disrupts the intron-exon junctions identified by Hwang et al. (1999) so that sequences 3' to the breakpoints are likely mistranslated. A complete alignment is available in the supplementary information at http://www.genetics.org/supplemental/. (B) A gar-3 reporter is expressed in neurons of the extrapharyngeal nervous system; in the pharyngeal muscles of the metacorpus, isthmus, and terminal bulb; and in pharyngeal neuron I3 (obscured by muscle staining). Bar, 50 um.

Picture from Ellenbecker, Mary et al. (2022) MicroPubl Biol

Figure 1. Mass spectrometric analysis of C elegans embryo after dlc-1 knock down:A-D) Immunostaining of control or dlc-1(RNAi) embryos. P granule protein PGL-1 is red and DNA is labeled with DAPI (blue). (A, B) 4 cell embryos (C, D) ~40 cell embryos. Images were acquired using a confocal microscope. Scale bars are 10 μM. Dotted lines outline embryos, which are oriented with anterior to the left and posterior to the right in this and subsequent figures. P granules are larger and restricted to the posterior cell of the 4 cell embryo and perinuclear in the 40 cell embryo in control RNAi (A, C) but mis-localized, smaller and dispersed after dlc-1(RNAi) (B, D). E) Volcano plot of mass spectrometric analysis of control RNAi vs. dlc-1(RNAi) treated C. elegans embryos. &#x2011;log10(Padj) (y-axis) is plotted against the average log2 fold change in protein level from 5 biological replicates (x-axis). Vertical black lines denote log2-fold change of -1 and 1 while the horizontal black line indicates an adjusted P-value of 0.01. Blue or red color indicates a significant fold change: blue are significantly decreased 2 fold or more; Padj <0.01, red are significantly increased 2 fold or more; Padj <0.01. As expected, DLC-1 protein levels are significantly decreased in dlc-1(RNAi) embryos and the PERM proteins were chosen for follow up analysis (enlarged blue points). Enlarged red points correspond to significantly increased proteins involved in stress response pathways, enlarged yellow points are P granule proteins and the enlarged green points are nucleoporin proteins. F) Relative protein levels of the core P granule proteins (corresponding to enlarged yellow dots in Figure 1E). dlc-1(RNAi) values were scaled to the mean of the control (from 5 replicate values), which was set to 1. *-Padj<0.01. G) Relative protein levels of the nucleoporin proteins previously shown to be important for P granule integrity (Voronina and Seydoux 2010). *-Padj<0.01. H) Relative protein levels of PERM-1/2 proteins. *-Padj<0.01. I-K) Live imaging of control RNAi, perm-2(RNAi) or perm-4(RNAi) treated embryos. (Ii and Iii-Ki and Kii) 2-4 cell embryos. (Iiii and Iiv-Kiii and Kiv) ~40 cell embryos. (Ii and Iiii -Ki and Kiii) White dashed lines outline embryos, GFP-tagged P granule protein PGL-3 is green and DNA is labeled with Hoechst 33342 (blue). Scale bars are 10 μM. (Iii and Iiv-Kii and Kiv) Brightfield images of embryos outlined by black dashed lines. Scale bars are 10 μM. Notice that Hoechst 33342 is able to penetrate the nematode eggshell only after perm-2 or perm-4 knockdown (compare Ii to Ji and Ki and Iiii to Jiii and Kiii).