Figure 2. Kinetics of OLD-1::GFP expression after environmental stressors and during aging.(a) The structure of the OLD-1::GFP reporter construct. (b) Photographs showing the anterior region of the OLD-1::GFP reporter worms unstressed or stressed by heat, starvation, and UV light (young adult hermaphrodites, 4 days after egg lay). (c) Photographs showing the expression patterns in old worms for wild-type and
daf-16(
m26). The bottom row shows Nomalski (left) and fluorescence (right) images of the anterior region of a typical OLD-1::GFP;
daf-16(
m26) worm. (d) Quantitative measures of expression during aging. (e) Survival of the OLD-1::GFP transgenic worms used in (d). Survival of the wild-type worms was shown as a control. (f-h) Quantitative measures of expression after exposure to (f) heat, (g) UV, and (h) starvation. The keys for (f)-(h) are the same as that for (d). Filled circles represent OLD-1::GFP, and open circles represent OLD-1::GFP;
daf-16(
m26).