(H) The GFP fusion forms of ANOH-1b but not ANOH-1a are localized to the plasmamembrane. GFP (a, c, e, g) and DIC (b, d, f, h) images of the tails of
mec-4(
e1611dm) L1-stage larvae. Arrowheads in (a, b, e, f) label necrotic cells on which ANOH-1b is observed on the cell surface. Arrows in (c, d, g, h) label necrotic cells in which ANOH-1a is observed on the nuclear surface. All GFP reportersare expressed under the Pmec-7 promoter control.