Picture from Wang H et al. (2013) Curr Biol "Neuropeptide secreted from a pacemaker activates neurons to control a rhythmic ...."

(C) The expression pattern of snt-2. Representative fluorescent image of an adult expressing a snt-2 transcriptional reporter, in which GFP is driven by the snt-2 promoter fragment (4,177 bp, from 792 bp upstream of ATG to 33 bp of the second exon of snt-2 gene). Arrowheads indicate fluorescence in head and tail.

Picture from Agostoni E et al. (1999) Gene "Identification and characterization of a new member of the gas3/PMP22 gene ...."

Fig. 5. Developmental Northern blot analysis of C01C10.1b and C01C10.1a. The C01C10.1b transcript, at about 1150 bp, and the 950-bp transcript of C01C10.1a are present in all developmental stages. The C. elegans initiation factor was used to visualize the relative amount of loaded mRNA.

Picture from Fay, Samuel F. et al. MicroPubl Biol

Figure 1. Summary of CRISPRcruncher: (A) Example CRISPRcruncher output displaying options for introducing restriction sites (>6 bp) within the first 33 coding nucleotides of CELE_F54B11.2. For simplicity, only 17/65 lines of output are shown. Red letters indicate changes from the input sequence; bold letters indicate the newly introduced endonuclease recognition site. 'Strand' column indicates whether the top (shown) or bottom strand contains the recognition motif; both indicates that the motif is an inverted repeat (a.k.a. palindrome). (B-G) Analyses of site frequencies for five randomly selected genes on the X chromosome. The first 99 coding nucleotides within the first exon of each gene were examined either as a contiguous sequence (B,C) or as three nonoverlapping 33-bp segments (D-G). 'Total Reported REs' indicates the complete set of REs, including isoschizomers and other categories of related motifs, capable of cleaving at the identified new sites. 'Distinct Sequences' indicates the total number of distinct new 33-bp or 99-bp sequences identified by CRISPRcruncher. Parameters were set to identify new restriction sites of 4 bp or greater (>4 cutter; red circles) or 6 bp or greater (>6 cutter; blue circles). (H) Percent of palindromic versus non-palindromic restriction motifs for the five 99-bp sequences. For supporting information, see Extended Data.

Picture from Zheng Q et al. (2002) J Biol Chem "Molecular cloning and characterization of a novel alpha ...."

Figure 6. The relative RT-PCR of CE2FT-1 mRNA from C. elegans at different stages. A, total C. elegans RNA from the populations of L1, L2-L4, and adult stage were prepared and used as templates in first strand cDNA synthesis. A 500-bp positive control from the RT-PCR kit was used as a control for the RT-PCR method (Line Positive Control). The arrows indicate the expected size of alpha-actin and CE2FT-1. Size markers in bp are indicated. B, the quantitative real-time RT-PCR of CE2FT-1 mRNA from C. elegans at different stages. Total C. elegans RNA from the populations of L1, L2-L4, and adult stage were prepared and used as templates in first strand cDNA synthesis. A 106-bp cDNA was amplified. The expression level was indicated by threshold cycles. C, confirmation of a single product of amplification in quantitative real time RT-PCR was performed on 1.2% agarose gel. A 106-bp cDNA fragment was amplified from CE2FT-1, and a 100-bp cDNA fragment was amplified from actin. Size markers in bp are indicated.

Picture from Barrios L et al. (1989) J Mol Biol "Genomic organization of the glyceraldehyde-3-phosphate dehydrogenase gene ...."

Figure 8. Ontogeny of mRNAs for gpd-4 and gpd-2. 10 g of total embryonic (E) and postembryonic larval (L4) mRNAs were dot blotted on to nitrocellulose paper and probed with gpd-2 and gpd-4-specific probes. The probe for gpd-2 was prepared using pSS1.3 (Fig. 3) derived from an M13 phage DNA using an oligonucleotide primer located 196 bp from its termination codon, which extended to a HaeIII site 5 bp before its stop codon. Since the 5' end of gpd-3 obtains a trans-spliced leader sequence (see Results and Discussion) this probe is specific for gpd-2 only. The gene-specific probe for gpd-4 was prepared using pB9 (Fig. 1(b)), an oligonucleotide 164 bp downstream from its termination codon, which was then extended to a HaeIII site located 10 bp beyond its stop codon.

Picture from Jiu Y et al. (2012) PLoS One "Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate ...."

Figure S1 Confocal image of adult hermaphrodites expressing Psec-6::GFP under the 2065 bp promoter (Ex[Psec-6::GFP; pRF4]). Left is anterior. Scale bar, 100 um.

Picture from Jiu Y et al. (2012) PLoS One "Exocyst subunits Exo70 and Exo84 cooperate with small GTPases to regulate ...."

Figure 1. Expression patterns of exocyst subunit exoc-7 in C. elegans. Confocal images of adult hermaphrodites expressing Pexoc-7::GFP under the 2954 bp promoter (Ex[Pexoc-7::GFP; pRF4]).

Picture from Helwick K et al. (2024) MicroPubl Biol "Reciprocal restriction fragment length polymorphism (RFLP) analysis reveals ...."

Figure 1. Reciprocal RFLP analysis of C. briggsae lines: Each DNA sample (HK104 x AF16 cybrid lines FV524-FV535, positive control parental strains AF16 and HK104, and negative control water) was PCR amplified by the two RFLP primer pairs at left (top row cb18178; bottom row cb34440). After PCR, an aliquot of each amplicon was digested with the restriction enzyme shown at left (SexAI or EcoNI). The undigested and digested samples were loaded into adjacent wells (- indicates absence of restriction enzyme; + indicates presence of restriction enzyme). M: molecular weight ladder; some bands are labeled at right (bands are in 100 bp increments from 100-1,000 bp). Electrophoresis occurred on one agarose gel and the left and right halves were imaged separately; a single M lane in the middle was acquired in both images to allow accurate alignment of the images. The positive control templates establish the expected digest band sizes. For cb18178, both AF16 and HK104 mtDNA produce 800 bp mtDNA amplicons; only HK104 digests into 650+150 bp products. For cb34440, both AF16 and HK104 produce 1,445 bp mtDNA amplicons; only the AF16 amplicon digests into 865+580 bp products. Thus, for cb18178 RFLP analysis, presence of any digest band indicates the presence of paternal mtDNA amplicons. For cb34440 RFLP analysis, presence of any digest band indicates the presence of maternal mtDNA amplicons. When both RFLP assays for a line produce digest bands, this indicates the line is heteroplasmic.

Picture from Brozova E et al. (2006) Mech Dev "NHR-40, a Caenorhabditis elegans supplementary nuclear receptor, regulates ...."

Fig. 5. Expression of nhr-40::gfp regulated by promoter region 1. Promoter region 1 includes 2833 bp upstream of the initiation codon (ATG) of exon 1 of transcript nhr-40a and nhr-40b (construct #4586). Expression is first detected at the 1.5-fold stage of embryogenesis (A) and continues during larval stages to adulthood (B-D). Strong expression is observed in the anterior pharynx (arrow) and scattered head neurons (C) and in the rectal gland cells (D, arrow). Expression from truncated constructs #6011 (1013 bp) and #6059 (682 bp) is indistinguishable and is observed in neurons (E, axon marked by arrowhead, ventral neuronal cord marked by arrow) and uterine muscles (F) of young adults. Scale: 20 um.

Picture from Tabarraei H et al. (2023) MicroPubl Biol "Investigation of PAL-1 requirement in C. elegans physiology using the ...."

Figure 1. Effect of PAL-1 auxin-inducible degradation on C. elegans physiology:(a) Schematic of AID*::3xFLAG::wrmScarlet knock-in at the pal-1 locus (referred to as PAL-1::degron), and primers used to genotype for cassette insertion. Scale bar indicates 500 base pair. (b) PCR gel to illustrate wildtype (512 bp), heterozygote knock-in (512 bp and 881 bp), and homozygous knock-in (881 bp) animals . All 3 primers were used in a single PCR to differentiate the 3 possible genotypes. The P1-P3 combination producing 1406 bp is not visible in the homozygous PAL-1::degron strain likely due to the preferential amplification of a shorter PCR product from P1-P2. Banding patterns observed below the 500 bp are likely due to non-specific amplification or primer dimers. (c) Effects of ethanol and auxin treatment on body length of PAL-1::degron C. elegans (MWU206) starting from L1. N=98-116 animals scored per condition. The scatter dot plot line indicates mean ± SD. (d) The Nob phenotype was observed in a small percentage (<1%) of auxin-treated MWU206 animals, the scale bar is 500 µm. Effects of ethanol and auxin treatment on (e) egg hatch rate of MWU206 (N= 7 replicates per condition with 36-91 offspring/eggs scored per replicate; scatter dot plot line indicates mean ± SD.), (f) MWU206 lifespan when fed with empty vector (EV) or ccf-1 RNAi bacteria grown on ETOH or auxin from L1; N > 125 animals were scored for each condition.