Fig. 3. Expression pattern and rescue of
cwn-2. (A-C)DIC images (top) and GFP images (bottom) showing expression of
cwn-2::SL2::GFP. (A)Comma stage C. elegans embryo. Arrow indicates approximate location of the developing nerve ring. (B)Adult. Arrowhead, body wall muscle; arrows, intestine; P, pharynx. (C)High magnification of adult head. Arrow, SMD neuron; P, pharynx. Scale bars: 10μm. (D)Transgenic rescue of
cwn-2 nerve ring defects by promoter::cDNA fusions. Nerve ring defects in each strain were scored at the L4 stage and normalized to defects in control siblings without the transgene (see Materials and methods). (E)Transgenic rescue of
cwn-2 nerve ring defects by embryonic heat shock-induced
cwn-2 expression. Controls are heat shocked
cwn-2 animals without the hs::
cwn-2 transgene. In D and E, error bars represent the s.e. of proportion. Asterisks indicate values different from controls at P<0.01 by Student's t-test.