Picture from Yamauchi S et al. (2007) Biochem Biophys Res Commun "Differential expression pattern of UBX family genes in Caenorhabditis ...."

Expression of ubxn-1. Two micrograms of total RNA prepared from each developmental stage was used for RT reaction.

Picture from Yamauchi S et al. (2007) Biochem Biophys Res Commun "Differential expression pattern of UBX family genes in Caenorhabditis ...."

Expression of ubxn-4. Two micrograms of total RNA prepared from each developmental stage was used for RT reaction.

Picture from Yamauchi S et al. (2007) Biochem Biophys Res Commun "Differential expression pattern of UBX family genes in Caenorhabditis ...."

Expression of ubxn-2. Two micrograms of total RNA prepared from each developmental stage was used for RT reaction.

Picture from Yamauchi S et al. (2007) Biochem Biophys Res Commun "Differential expression pattern of UBX family genes in Caenorhabditis ...."

Expression of ubxn-6. Two micrograms of total RNA prepared from each developmental stage was used for RT reaction.

Picture from Yamauchi S et al. (2007) Biochem Biophys Res Commun "Differential expression pattern of UBX family genes in Caenorhabditis ...."

Expression of ubxn-5. Two micrograms of total RNA prepared from each developmental stage was used for RT reaction.

Picture from Yamauchi S et al. (2007) Biochem Biophys Res Commun "Differential expression pattern of UBX family genes in Caenorhabditis ...."

Expression of ubxn-3. Two micrograms of total RNA prepared from each developmental stage was used for RT reaction.

Picture from Chen PJ et al. (2001) Genetics "Specification of germ cell fates by FOG-3 has been conserved during nematode ...."

(B) Quantitative RT-PCR analysis of fog-3 expression in C. remanei. RNA was prepared from single worms of the indicated ages and used for RT-PCR analysis of fog-3 expression. The number of cycles was set so that a twofold dilution of the male samples yielded a corresponding decrease in the intensity of the resulting band.

Picture from Pispa J et al. (2008) BMC Dev Biol "C. elegans dss-1 is functionally conserved and required for oogenesis and larval ...."

dss-1 mRNA is expressed at different larval ages (L1-L4) and in adult worms (ya, young adult) as shown by RT-PCR. act-4, actin(M03F4.2).

Picture from Park YS et al. (2003) Biochem Biophys Res Commun "Alternative splicing of the muscarinic acetylcholine receptor GAR-3 in ...."

Fig. 2. RT-PCR analysis of the gar-3 splice variants. RT-PCR was performed using the stage-specific RNA as a template. The PCR products were analyzed on a 1.2% agarose gel. The expected sizes of the PCR products for gar-3a and gar-3b are 825 and 747 bp, respectively. E, embryo; L1-L4, first to fourth larval stages; and A, young adult.

Picture from Zheng Q et al. (2002) J Biol Chem "Molecular cloning and characterization of a novel alpha ...."

Figure 6. The relative RT-PCR of CE2FT-1 mRNA from C. elegans at different stages. A, total C. elegans RNA from the populations of L1, L2-L4, and adult stage were prepared and used as templates in first strand cDNA synthesis. A 500-bp positive control from the RT-PCR kit was used as a control for the RT-PCR method (Line Positive Control). The arrows indicate the expected size of alpha-actin and CE2FT-1. Size markers in bp are indicated. B, the quantitative real-time RT-PCR of CE2FT-1 mRNA from C. elegans at different stages. Total C. elegans RNA from the populations of L1, L2-L4, and adult stage were prepared and used as templates in first strand cDNA synthesis. A 106-bp cDNA was amplified. The expression level was indicated by threshold cycles. C, confirmation of a single product of amplification in quantitative real time RT-PCR was performed on 1.2% agarose gel. A 106-bp cDNA fragment was amplified from CE2FT-1, and a 100-bp cDNA fragment was amplified from actin. Size markers in bp are indicated.