Picture from Stear JH et al. (2004) Mol Biol Cell "The Caenorhabditis elegans kinetochore reorganizes at prometaphase and in ...."

Figure 1. Localization of centromere and kinetochore components at prophase and metaphase. Embryos were stained with antibodies against HCP-3/CeCENP-A (green) and HCP-1/CeCENP-F (red). DNA was visualized using DAPI (blue). These images were collected from one-cell embryos. At prophase, centromere and kinetochore proteins colocalize to parallel lines running the length of each chromosome (a-c). At metaphase, all known components of the centromere and kinetochore are present on the poleward faces of the metaphase plate, as seen from the perpendicular perspective (d-f). Bar, 1 um.

Picture from Stear JH et al. (2002) Genes Dev "Characterization of HCP-6, a C. elegans protein required to prevent chromosome ...."

Figure 2. Localization of HCP-6 throughout the cell cycle. (A-P) Wild-type embryos showing DNA (blue), anti-HCP-6 (red), or anti-HCP-3 (green) staining, or merged images. Colocalization between HCP-6 and HCP-3 is shown as yellow. (A-D) At interphase, this antigen is visible as punctate dots distributed throughout the nucleus. (E-H) In prophase nuclei, HCP-6 forms two parallel lines on individual prophase chromosomes, a pattern suggesting centromere localization. (I-L) At metaphase, the HCP-6 protein is present on the poleward faces of the metaphase plate. (M-P) During anaphase, HCP-6 remains associated with the separating groups of sister chromatids.

Picture from Stear JH et al. (2002) Genes Dev "Characterization of HCP-6, a C. elegans protein required to prevent chromosome ...."

Figure 2. Localization of HCP-6 throughout the cell cycle. (A-P) Wild-type embryos showing DNA (blue), anti-HCP-6 (red), or anti-HCP-3 (green) staining, or merged images. Colocalization between HCP-6 and HCP-3 is shown as yellow. (A-D) At interphase, this antigen is visible as punctate dots distributed throughout the nucleus. (E-H) In prophase nuclei, HCP-6 forms two parallel lines on individual prophase chromosomes, a pattern suggesting centromere localization. (I-L) At metaphase, the HCP-6 protein is present on the poleward faces of the metaphase plate. (M-P) During anaphase, HCP-6 remains associated with the separating groups of sister chromatids. (Q-S)hcp-3(RNAi) embryos stained with anti-HCP-6 (red). In the absence of HCP-3, HCP-6 does not assemble onto mitotic chromosomes properly. Bars, 1 μm.