Picture from Crook, Matt et al. (2021) MicroPubl Biol

Figure 1 : A let-23(sa62)gf mutation in C. elegans strongly suppresses a pnc-1(pk9605) egg-laying (Egl) phenotype, where adult pnc-1(pk9605) hermaphrodites form "bags of worms" by four days post L4>adult molt. A) A gain-of-function mutation in let-23 is sufficient to rescue the pnc-1 Egl phenotype, but gain-of-function mutations in downstream genes let-60 and/ or itr-1 are not. B) Phosphatidylcholine synthesis is neither required nor sufficient for the let-23(ga62)gf rescue of the pnc-1 Egl phenotype. WT = wild-type C. elegans N2 Bristol strain. Error bars are 95% confidence intervals, with proportion and sample size in the data labels. Proportions were analysed by pairwise.prop.test in R with Holm p value adjustment; *** and n/s represent p<0.0001 and non-significant, respectively.

Picture from Toraason E et al. (2024) MicroPubl Biol "In vivo neuron-specific expression of C. elegans reprogramming ...."

Figure 1. Effect of neuronal OSK on C. elegans lifespan and cognitive aging: A) A doxycycline-inducible genetic construct enables neuronal expression of C. elegans OSK factor orthologs and GFP with temporal control. Images displayed are maximum intensity projections of representative head and tail neurons following exposure to control buffer or doxycycline (see Methods). Scale bars indicate 50μm.B) Lifespan assay (1 replicate) of CQ751 worms and wild-type non-array carrying siblings never exposed to doxycycline (control), exposed to doxycycline throughout adult life (continuous dox), or transiently exposed to doxycycline for 48h every 4 days (transient dox). P values were calculated by Log-Rank test with Bonferroni-Holm correction for multiple comparisons.C) Diagram of appetitive memory assay and timing at which CQ819 worms were exposed to doxycycline (24 hours beginning at adult day 2).D) Appetitive short-term memory assay of day 5 adult CQ819 worms exposed to doxycycline for 24 hours beginning at adult day 2. P values were calculated by ANOVA with Tukey's HSD tests. 1 representative replicate of n=2 independent experiments is displayed.E) Diagram of aversive memory assay and timing at which CQ819 worms were exposed to doxycycline (48 hours beginning at adult day 2).F) Aversive short-term memory assay (1 replicate) of day 5 adult CQ819 worms exposed to doxycycline for 48 hours beginning at adult day 2. P values were calculated by ANOVA with Tukey's HSD tests.G) 1% butanone chemotaxis assay (1 replicate) of day 5 adult CQ819 worms exposed to doxycycline for 48 hours beginning at adult day 2. P values were calculated by Student's t-test.H) 10mg/mL pyrazine chemotaxis assay (1 replicate) of day 5 adult CQ819 worms exposed to doxycycline for 48 hours beginning at adult day 2. P values were calculated by Student's t-test.In all panels, n.s. = p>0.05, * = p<0.05, ** =p<0.01, ***=p<0.001, **** = p<0.0001.