Picture from Schafer JC et al. (2003) Mol Biol Cell "XBX-1 encodes a dynein light intermediate chain required for retrograde ...."

Figure 2. Restoration of cilia in xbx-1 mutants by transgenic rescue. All panels are bright field images overlaid with fluorescent images to show dye-filling (red) and XBX-1:: YFP localization (yellow). (A) Six pairs of amphid neurons of wild-type N2 worms fill with the fluorescent dye DiD. (B) In contrast, xbx- 1(ok279) mutant worms fail to take up the dye. (C) Transgenic expression of xbx-1::yfp in xbx-1(ok279) mutant worms restores the ability to uptake fluorescent dye (red). The XBX-1::YFP protein is detected at the transition zones and within cilia on the ends of the amphid neurons (yellow, arrowhead). Anterior is toward the left in all panels.

Picture from Meissner B et al. (2011) PLoS One "Determining the sub-cellular localization of proteins within Caenorhabditis ...."

Figure 8. The GFP-tagged F21C10.10 protein co-localizes with the MitoTracker dye in mitochondria. Panel A shows the co-localization of the F21C10.10 GFP-tagged protein (green) with the MitoTracker dye (red) in mitochondria in the same body wall muscle cell. Panel B shows the GFP localization pattern of F21C10.10 (shown in green) in a body wall muscle cell in an animal from the DM7305 strain. Panel C shows the mitochondria staining with the MitoTracker dye (shown in red) in the same body wall muscle cell. The arrow points to a single mitochondrion. Bars represent 10 um.

Picture from Ferkey DM et al. (2007) Neuron "C. elegans G protein regulator RGS-3 controls sensitivity to sensory ...."

Figure S2. RGS-3 Expression Pattern in Head Sensory Neurons. RGS-3::GFP expression is seen in seven pairs of head sensory neurons (ASH, ADL, AWB, AWC, ASI, ASJ and ASK) as well as PHA and PHB in the tail (not shown). Top panel: The fluorescent dye DiD was used to identify dye-filling neurons. Middle panel: RGS-3::GFP expression is seen in all of the dye-filling neurons as well as AWC. Cell bodies are labeled. Bottom panel: Merged images. Reproducible RGS-3::GFP expression in ASH was confirmed, although the intensity of the DiD staining masks the GFP in this merged image. In all panels, the tip of the animal's nose is to the left.

Picture from Li C et al. (2016) PLoS Biol "MKS5 and CEP290 Dependent Assembly Pathway of the Ciliary Transition ...."

Fig 5. CDKL-1 and TMEM-138 require CEP-290 for their transition zone localisation and are functionally independent of the MKS or NPHP module.(A) GFP-tagged CDKL-1 (green) requires MKS-5 (dotted ellipse) but not other "core" MKS (MKS-2, TMEM-231) or NPHP (NPHP-4) module proteins for itsTZ localisation. XBX-1 is used to mark the basal body-transition fibres and axonemes. Scale bar, 4 um. (B) Dye-filling assays of head (amphid) and tail(phasmid) sensory neurons reveal that cdkl-1 does not genetically interact (i.e., shows synthetic dye-filling phenotype) with either MKS (mks-2) or NPHP(nphp-1) module mutants. cdkl-1 also does not show a genetic interaction with tmem-138. Scale bar, 40 um. (C) Both GFP-tagged CDKL-1 and TMEM-138depend on CEP-290 for their localisation to the TZ (dotted ellipse). Scale bar, 4 um. (D) Partial (weak) dye-filling phenotype in the cep-290;tmem-138 and cep-290;cdkl-1 double mutants (++) compared to wild-type and the individual mutants (normal dye-filling, +++)

Picture from Haynes CM et al. (2010) Mol Cell "The matrix peptide exporter HAF-1 signals a mitochondrial UPR by activating the ...."

(E) Fluorescent photomicrographs of CHO cells expressing GFP (upper panels) or GFP fused to aa 1-75 of HAF-1 (HAF-11-75::GFP, lower panels). The cells were costained with the vital dye MitoTracker, which stains mitochondria (middle panels).

Picture from Hagstrom KA et al. (2002) Genes Dev "C. elegans condensin promotes mitotic chromosome architecture, centromere ...."

(A-D) Wild-type embryo costained with SMC-4 antibody (green, A), DNA dye (red,B), and tubulin antibody (blue, C; merge inD). SMC-4 associates with condensed mitotic chromosomes within mitotic spindles, but not with interphase chromosomes. (Arrowhead) Mitotic nucleus; (arrow) interphase nucleus.

Picture from Garg P et al. (2022) MicroPubl Biol "DiI staining of sensory neurons in the entomopathogenic nematode Steinernema ...."

Figure 1 Optimized DiI staining labels amphid and inner labial neuron analogs of S. hermaphroditum:(A) Optimization of DiI head sensory neuron staining in S. hermaphroditum. The procedure was optimized for dye concentration (top 3 rows), buffers (middle 3 rows), and staining duration (bottom 4 rows) Living worms with at least one stained neuron and associated dendrite were scored as positive. P values are from the Fisher exact test. *: statistically significant. #: 0.5% Triton X-100 in M9. (B, C) Dye filling in the head neurons of C. elegans. Arrows indicate identified neurons. The anterior of the worm is to the left and in (B) dorsal is up and in (C) ventral is up. Scale bar, 20µm. (D, F) Dye filling in the head region of S. hermaphroditum, arrows indicate neurons identified (in parentheses) based on positional analogy to C. elegans counterparts. Scale bar, 20µm. (E, G) DIC (differential interference contrast) images of (D) and (F), respectively. Scale bar, 20µm. In (D, E) the anterior of the worm is to the top-left and the dorsal is bottom-left. Worm is lying in a twisted position on the agar pad. (F, G) the anterior of the worm is to the left and the dorsal is up. (H) Adult worm with dye filling (arrow) in the gut. The anterior of the worm is to the top-right and the dorsal is top-left. The dye filled gut is partly obscured by the gonad. Scale bar, 200µm. (I) DiI reliably fills the excretory pore (arrow). The anterior of the worm is to the left and dorsal is up. Scale bar, 20µm. (J) Infective juveniles (IJs) are dye-filling resistant. The staining seen at the posterior end of the animal is a shed cuticle from molting. Scale bar, 50µm. (H-J) Images were merged from DIC and DiI images. "V" Ventral, "D" Dorsal, "P" Posterior, "A" Anterior.

Picture from Oikonomou G et al. (2011) PLoS Biol "Opposing activities of LIT-1/NLK and DAF-6/patched-related direct sensory ...."

Figure 5. mom-4/TAK1 mutations suppress the loss of daf-6. (A) Dye filling in animals of the indicated genotypes (n$90). The alleles used are: daf-6(n1543), lit-1(ns132), mom-4(ne1539). daf-6 is marked with unc-3(e151) in all strains except for mom-4; daf-6. unc-3(e151) does not affect dye filling (unpublished data). Error bars, SEM. p value calculated using Chi-squared test. (B) Schematic of Wnt signaling during endoderm specification in C. elegans. In contrast to the LIT-1 MAPK module (red), Wnt signaling does not appear to be involved in amphid sheath channel formation (see text and Table S1).

Picture from Hagstrom KA et al. (2002) Genes Dev "C. elegans condensin promotes mitotic chromosome architecture, centromere ...."

Figure 2. SMC-4 colocalizes with MIX-1 at the poleward face of condensed mitotic chromosomes but not on X chromosomes. (A-E) The mitotic chromosome condensation cycle. Merged confocal images of wild-type embryonic nuclei costained with DNA dye (red) and tubulin antibody (green) show C. eleganschromosomes and microtubules throughout mitosis. (F-J) Merged confocal images of wild-type embryonic nuclei costained with SMC-4 antibody (green) and DNA dye (red); overlap appears yellow. (K-N) Early wild-type embryo costained with DNA dye (blue, K), MIX-1 antibody (red,L), and SMC-4 antibody (green, M). MIX-1 and SMC-4 colocalize in nuclei with condensed chromosomes (yellow when merged,N). At this early stage, dosage compensation is not active; MIX-1 and other dosage compensation proteins are not localized to the X chromosome. (O-Q) Metaphase chromosomes costained with DNA dye (blue), MIX-1 antibody (red, O), and SMC-4 antibody (green, P) show colocalization of MIX-1 and SMC-4 at the poleward face of condensed chromosomes (yellow when merged,Q). (R-T) Wild-type larva costained for DNA (blue), MIX-1 (red), and SMC-4 (green). MIX-1 and SMC-4 both stain nuclei of two mitotic cells (mit), but only MIX-1 shows the punctate subnuclear staining characteristic of dosage compensation proteins bound to hermaphrodite X chromosomes (X).

Picture from Schafer JC et al. (2003) Mol Biol Cell "XBX-1 encodes a dynein light intermediate chain required for retrograde ...."

Transgenic expression of xbx-1::yfp in xbx-1(ok279) mutant worms restores the ability to uptake fluorescent dye (red). The XBX-1::YFP protein is detected at the transition zones and within cilia on the ends of the amphid neurons (yellow, arrowhead). Anterior is toward the left in all panels.