Picture from Attix, Haley et al. (2021) MicroPubl Biol

Figure 1. Comparison of N2 and wild caught nematode early embryo divisions, 18S subunit gDNA amplification and sequencing analysis, with additional morphology comparison for identification: A. Embryonic cell divisions of N2 Bristol (common lab strain) and a wild caught Woods Hole (WH) strain. Early embryonic cells are labelled to highlight differences in cell division programs from one through 4 cell stages (Goldstein, 2001). Scale bar is ~10um. B. Electrophoresis gel of PCR product run with template gDNA extracted from N2 and WH strains to verify 18S ribosomal subunit band for sequencing (~884 bps). C. Sequence alignment of a conserved hairpin 17 region of the 18S sequence of N2 and the WH strain. A BLAST search identified the WH strain as Acrobeloides sp. D. Morphological comparison of N2 and WH strains shows an expected pronounced terminal bulb (tb) of the adult pharynx in both species. The anterior bulb (*) is noticeable in N2, but less pronounced in the WH strain as seen in previous characterization of Acrobeloides.