Picture from Chen P-J et al. (2000) Development "TRA-1A regulates transcription of fog-3, which controls germ cell fate in C. ...."

The period of high fog-3 expression coincides with spermatogenesis. Northern blot of N2 total RNA, isolated from different developmental stages. L1, first larval stage; yA, young adult; A, adult. The blot was probed with an anti-fog-3 probe (Chen et al., 2000) and then with a probe to the 26s ribosomal RNA, to test for loading errors.

Picture from Chen P-J et al. (2000) Development "TRA-1A regulates transcription of fog-3, which controls germ cell fate in C. ...."

Figure 2. The period of high fog-3 expression coincides with spermatogenesis. (A) Northern blot of N2 total RNA, isolated from different developmental stages. L1, first larval stage; yA, young adult; A, adult. The blot was probed with an anti-fog-3 probe (Chen et al., 2000) and then with a probe to the 26s ribosomal RNA, to test for loading errors. (B) Northern blot of him-5(e1490) total RNA, processed as described in A. These populations contain 30% males and 70% hermaphrodites (Hodgkin et al., 1979).

Picture from Larsen MK et al. (2006) Mol Biol Cell "MAA-1, a novel acyl-CoA-binding protein involved in endosomal vesicle ...."

Figure 3. MAA-1 localizes to endosomal and Golgi compartments. Photomicrographs of the intestines of worms viewed with confocal fluorescence microscopy. The five panels on the left show GFP fluorescence in worms expressing MAA-1::GFP fusion proteins. The five middle panels show staining of the same worms with markers for different membrane organelles. FM4-64 is a membrane-intercalating dye that labels early and late endosomes. LysoTracker labels late endosomes and lysosomes. An antibody that recognizes the ER retention signal HDEL was used to label the ER. An antibody that recognizes the Golgi protein GRASP55 was used to label Golgi. Staining with this antibody substantially overlaps that obtained with a marker for Golgi in C. elegans, alpha-mannosidase (Chen et al., 2006) (Supplemental Figure S2). Because the epitope from human GRASP55 used to raise the antibody is almost completely conserved in the worm orthologue, it is likely that the antibody recognizes worm GRASP55. RME-1 labels the ERC. The five panels on the right show merged images of the respective left and middle panels. Bar, 5 um. Selected images of worms at lower magnification are shown in Supplemental Figure S3.

Picture from Siete C et al. (2024) MicroPubl Biol "Immobilization of C. elegans with different concentrations of an ...."

Figure 1. Comparison of axonal transport of TIR-1:GFP in AWC axons of worms immobilized with different concentrations of tetramisole and polystyrene microbeads:(A) Representative kymographs of moving TIR-1::GFP in AWC axons of worms immobilized with 0.5-7.5 mM tetramisole (Tet) or microbeads. The line within two blue arrowheads represents a retrograde trafficking event toward the proximal axon (P) or the ­­­­­AWC cell body; the line between two red arrowheads represents an anterograde trafficking event toward the distal (D) axon. (B) The percentage of anterograde and retrograde trafficking events. The average number of trafficking events per animal imaged is 19 (0.5 mM Tet), 24 (1 mM Tet), 21 (2 mM Tet), 19 (7.5 mM Tet), and 13 (microbeads). Z-test was used to determine p-values. Error bars, SE of proportion. ns, not significant. (C) The average velocity of anterograde and retrograde trafficking events. Red and blue lines indicate the comparison of anterograde and retrograde velocities, respectively. Student's t-test was used to determine p-values. Error bars, SEM. ns, not significant. The significance level 0.01 was adjusted to 0.0025 (0.01/4) using the Bonferroni method (Chen, Feng, & Yi, 2017) in (B) and (C).

Picture from Lanjuin A et al. (2003) Dev Cell "Otx/otd homeobox genes specify distinct sensory neuron identities in C. ...."

Figure 2. Genomic Structure and Expression Patterns of ceh-36 and ceh-37. (A) ceh-36 and ceh-37 genomic sequences are adjacent on the cosmid C37E2. Shown are the genomic structures of both genes with the positions of the mutations and the extent of deletions marked. Filled boxes indicate the homeodomains.(B) Alignment of the homeodomains of CEH-36, CEH-37, C. elegans TTX-1 (GI:25154683), rat OTX1 (GI:6981313), rat OTX2 (GI:27699028), and Drosophila OTD (GI:8311). The alignment was performed using the ClustalW algorithm (Higgins et al., 1996). Identical residues are boxed in black; conserved residues are boxed in gray. The positions of the helices are overlined. The asterisk indicates the characteristic Lys at position 9 of the third helix. The three amino acid insertion in the third helix of CEH-36 is marked with a dashed underline.(C) A ceh-37::gfp fusion gene is expressed in the AWB neurons of 3-fold embryos. Arrow points to the characteristic forked cilia of an AWB neuron. The corresponding DIC image is shown at top.(D) Expression of a ceh-37::gfp fusion gene is lost in head neurons and is maintained in the excretory cell (arrow) in adult animals. DIC image is at top.(E) A gfp-tagged rescuing ceh-36 transgene is expressed in the AWC and ASE neurons in adult animals (green). GFP is localized to the nuclei. These animals also express odr-1::dsRed strongly in the AWC and weakly in the AWB olfactory neurons (red). Schematic of the expression pattern is shown at top. Scale bar, 10 um.