Picture from Meissner B et al. (2011) PLoS One "Determining the sub-cellular localization of proteins within Caenorhabditis ...."

Figure 5. Sub-cellular localization patterns for some category 5, 6 and 7 GFP-tagged proteins. All 5 panels show single symmetrical, spindle-shaped body wall muscle cells exhibiting GFP-tagged protein localization. The category 5 protein, T03G6.3, (A), is most likely a component of the sarcoplasmic reticulum. The category 6 protein, T22A4.3, (B), is found in the dense bodies, thick filaments and/or M-lines and the ER/SR. The category 7 proteins, Y71F9B.3 (C), ZK637.3 (D) and R07E5.7 (E), all localize to the muscle cell membrane in various patterns. Bars represent 10 um.

Picture from Gonzalez-Sandoval A et al. (2015) Cell "Perinuclear Anchoring of H3K9-Methylated Chromatin Stabilizes Induced Cell ...."

Figure 2. Perinuclear CEC-4 Localization Is Independent of H3K9 Methylation, and Varies from Tissue to Tissue(A) Single plane images of indicated embryo stages expressing CEC-4-mCh.(B) SR-SIM microscopy of CEC-4-mCh transgenic embryos, counterstained for nuclear pores, lamin or mCherry. Embryo sections and single nuclear planesshown.(C) Z-projection of CEC-4-mCh in met-2 set-25 mutant background; images of mCherry alone and merged with gwIs4 GFP-LacI signal are shown. Insets: singleplain nuclei. Quantification of array distribution, n = foci scored.(D) Single plane confocal images of CEC-4- and EMR-1-mCh transgenic L1 larvae; scheme of L1 worm color-coded by tissue, M: muscle, I: intestine, H: hypoderm. Measured mCh signal intensity displayed as box plots in a.u. as in Figure 1. Black circles = outliers, n = number of nuclei per tissue; pair-wise comparisons for * and **p value < 0.001 in Wilcoxon test. Scale bar, 5 um in whole/section embryos and larvae; 2um in single nuclei/insets.See also Figure S2.

Picture from Jaramillo-Lambert A et al. (2010) Curr Biol "Meiotic errors activate checkpoints that improve gamete quality without ...."

Figure 5. The Proapoptotic Machinery Is Expressed in the Male Germline during Meiotic Prophase, but CED-3 Caspase Is Not Activated. (A) Pathway of germ cell apoptosis in C. elegans [53].(B) CED-9 inactivation does not induce apoptosis in the male germline. Number of apoptotic nuclei per gonad arm measured by AO staining 24 hr post L4 stage (and 24 hr post IR) is indicated. The number of germlines scored for each genotype is in parenthesis. The data shown are means ± SEM.(C) CED-4 and SIR-2.1 are expressed in both hermaphrodite and male germlines, but no SIR-2.1 translocation occurs in male germ cells. Images show immunolocalization of CED-4 (green) and SIR-2.1 (red) counterstained with DAPI (blue) in pachytene nuclei from wild-type and zim-1(tm1813) hermaphrodite and male germlines. Arrowheads point to nuclei with more intense CED-4 staining. Scale bars represent 5 μm.(D) CED-3 is expressed in hermaphrodite and male germlines, but levels do not increase under checkpoint-activating conditions in the male germline. Images show CED-3 immunolocalization (green) in pachytene nuclei stained with DAPI (blue) of adult worms (24 hr post L4). Arrowheads indicate apoptotic nuclei that lack CED-3 staining presumably because they have proceeded to a later stage. Scale bars represent 5 um.(E) Males do not activate CED-3 caspase. Caspase activity was monitored by fluorescence microscopy following microinjection of SR-FLICA inhibitor in hermaphrodite and male germlines of WT, zim-1(tm1813), and zim-1(tm1813);ced-3(RNAi) worms. Arrowheads indicate some of the caspase-positive cells. Scale bars represent 10 μm.See also Figure S3.