Picture from Kasper DM et al. (2014) Dev Cell "The C. elegans SNAPc component SNPC-4 coats piRNA domains and is globally ...."

Supplemental Figure 1. SNPC-4:GFP rescues the lethality of snpc-4 (tm4568) mutants (related toFigure 1). (A) Annotated gene model for SNPC-4, the C. elegans ortholog to mammalian SNAPC4. The snpc4 (tm4568) mutant allele contains a deletion that removes portions of the Myb-like/ SANT DNA- and chromatinbinding domains (PFAM ID: PF13921 (blue) and PFAM ID: PF00249 (orange)), resulting in a premature stop codon (*TGA). (B) SNAPC4, the major DNA-binding subunit of the mammalian SNAPc complex (blue), binds the external promoter of snRNA genes at the proximal sequence element (PSE). (top) SNAPc with the Pol II pre-initiation complex consisting of Pol II (purple) and the multi-subunit TFII complex (pink) promotes the transcription of U1-U5 snRNA genes. (middle) SNAPc with the Pol III pre-initiation complex consisting of Pol III (green) and the multi-subunit TFIIIB complex (light green) initiates the transcription of U6 snRNA genes. (bottom) Independent of SNAPc, Pol III machinery consisting of Pol III, TFIIIB complex, and TFIIIC complex (magenta) binds the internal promoter of tRNA genes at the A box and B box motif to promote their transcription. Images adapted from (Jawdekar and Henry, 2008; (White, 2011). (C) Embryonic lethality of the snpc-4(tm4568) mutant is rescued by SNPC-4:GFP expression. The snpc-4 mutant is balanced by hT2. Parental strains containing the hT2 balancer or SNPC-4:GFP transgene alone are shown for comparison. Average number of dead embryos was assessed for at least 4 adult hermaphrodites per genotype. Error bars represent SD. (D-F) Diffuse SNPC-4:GFP nuclear localization in hermaphrodite somatic tissues, such as the head (D), vulva (E), and tail (F). Brackets indicate representative regions in which somatic, nuclear SNPC4:GFP is detected. (G) SNPC-4:GFP localizes to sub-nuclear foci in the male germ line (dotted outline). Depicted germ line is in a him-8 mutant background. Arrows point to representative examples of nuclei with SNPC-4:GFP expression. Scale bars are 10 um.

Picture from Kasper DM et al. (2014) Dev Cell "The C. elegans SNAPc component SNPC-4 coats piRNA domains and is globally ...."

Supplemental Figure 1. SNPC-4:GFP rescues the lethality of snpc-4 (tm4568) mutants (related toFigure 1). (A) Annotated gene model for SNPC-4, the C. elegans ortholog to mammalian SNAPC4. The snpc4 (tm4568) mutant allele contains a deletion that removes portions of the Myb-like/ SANT DNA- and chromatinbinding domains (PFAM ID: PF13921 (blue) and PFAM ID: PF00249 (orange)), resulting in a premature stop codon (*TGA). (B) SNAPC4, the major DNA-binding subunit of the mammalian SNAPc complex (blue), binds the external promoter of snRNA genes at the proximal sequence element (PSE). (top) SNAPc with the Pol II pre-initiation complex consisting of Pol II (purple) and the multi-subunit TFII complex (pink) promotes the transcription of U1-U5 snRNA genes. (middle) SNAPc with the Pol III pre-initiation complex consisting of Pol III (green) and the multi-subunit TFIIIB complex (light green) initiates the transcription of U6 snRNA genes. (bottom) Independent of SNAPc, Pol III machinery consisting of Pol III, TFIIIB complex, and TFIIIC complex (magenta) binds the internal promoter of tRNA genes at the A box and B box motif to promote their transcription. Images adapted from (Jawdekar and Henry, 2008; (White, 2011). (C) Embryonic lethality of the snpc-4(tm4568) mutant is rescued by SNPC-4:GFP expression. The snpc-4 mutant is balanced by hT2. Parental strains containing the hT2 balancer or SNPC-4:GFP transgene alone are shown for comparison. Average number of dead embryos was assessed for at least 4 adult hermaphrodites per genotype. Error bars represent SD. (D-F) Diffuse SNPC-4:GFP nuclear localization in hermaphrodite somatic tissues, such as the head (D), vulva (E), and tail (F). Brackets indicate representative regions in which somatic, nuclear SNPC4:GFP is detected. (G) SNPC-4:GFP localizes to sub-nuclear foci in the male germ line (dotted outline). Depicted germ line is in a him-8 mutant background. Arrows point to representative examples of nuclei with SNPC-4:GFP expression. Scale bars are 10 um.