Figure S3. SOL-2 is co-expressed with GLR-1 and SOL-1 in the command interneurons. Supplemental data associated with Figure 3. (A) Confocal images of the head and tail regions of transgenic worms that expressed Psol-2::gfp with either Pglr-1::mCherry (top), Psol-1::mCherry (middle), or Pnmr- 1::mCherry (bottom). The arrow indicates the AVA command interneurons, which were used in all in vivo electrophysiology experiments. Scale bars represent 10 um. (B) Current evoked by 3 mM glutamate in AVA of transgenic worms that overexpressed either GFP::SOL-1 and GFP::SOL-2, or N-YFP::SOL-1 and C-YFP::SOL-2 (top), and quantification of peak glutamate-gated current (bottom). Cells were held at -60 mV and n=3 for both genotypes. (C) Quantification of GLR-1::mCherry puncta fluorescence intensity, density and width in the AVA processes of transgenic wild-type (WT) worms and worms that overexpressed N-YFP::SOL-1 and C-YFP::SOL-2. n=10 worms for both genotypes; *, p<0.05. (D) Quantification of SOL-1/SOL-2 BiFC puncta fluorescence intensity, density and width in the AVA processes of transgenic wild-type (WT) worms and
glr-1(
ky176) mutants. n=11 worms for both genotypes; *, p<0.01. Error bars indicate SEM.