Figure 5. EGRH-1 antibody staining and EGRH-1::GFP expression in the somatic gonad and intestine. EGRH-1 antibody staining (top panels) and EGRH-1::GFP expression (bottom panels) in nuclei of the distal tip cell (DTC) and sheath cells of the somatic gonad and intestinal cells. Arrowheads mark representative EGRH-1-containing nuclei. White lines point out oocyte nuclei lacking detectable EGRH-1 staining. For anti-EGRH-1, antibody-stained and DAPI-stained images are shown individually and merged. For EGRH-1::GFP, GFP fluorescence and DIC images are shown individually and merged. Distal tip cell staining with EGRH-1 antibody colocalized with
lag-2::gfp expression, which marks this cell type (data not shown) (Siegfried and Kimble, 2002). Scale bars: 40 μm.