Picture from Head BP et al. (2011) Mol Biol Cell "A novel mitochondrial outer membrane protein, MOMA-1, that affects cristae ...."

Figure 3: Localization of MOMA-1 to the mitochondrial outer membrane. (A-F) Staining of C. elegans gonads. (G-L) Staining of C. elegans embryos with MOMA-1 antibody (green) and propidium iodide (red in A, B, and G-I) or cytochrome c antibody (red in C-F and J-L). Strains are indicated above the panels, and the combinations of stains are shown on the left, except for F and K, which were stained with cytochrome c antibody but not with MOMA-1 antibody to control for bleed-through. The scale bar is 10 μm for A and B, 20 μm for C-F, and 10 μm for G-L. (M) Western blots of extracts from wild-type (lane 1) and moma-1(tm1912) (lane 2) animals probed with antibodies against tubulin or MOMA-1. Size markers are in kDa. (N) Subcellular distribution of MOMA-1 determined by differential centrifugation. Lanes were loaded with total extracts (T), with 14,000 g pellets (P2), and with 14,000 g supernatants (S2; P2 and S2 fractions were volume equivalents). Blots were probed with EAT-3, tubulin, and MOMA-1 antibodies as indicated. The distributions were quantified with densitometry of P2 and S2 fractions: 88% of EAT-3, 12% of tubulin, and 98% of MOMA-1 is in the mitochondrial pellet. (O) Protease protection experiment to determine the submitochondrial localization of MOMA-1. Mitochondria were isolated by differential centrifugation and treated with 0 (lanes 1 and 4), 30 (lanes 2 and 5), and 60 (lanes 3 and 6) μg/ml proteinase K, without (lanes 1-3) or with (lanes 4-6) 1% Triton X-100. Blots were probed with antibodies for EAT-3 (an inner membrane marker), MOMA-1, MFF-1 (an outer membrane marker), and F1beta subunit of the ATP synthase complex (a mitochondrial matrix marker). MOMA-1 is digested when no detergent is added, like MFF-1, while EAT-3 and F1beta are protease protected. For unknown reasons, an intermediate cleavage product of MFF-1 is detected when Triton is added to the protease digestion but not when Triton is omitted.

Picture from Wang H et al. (2013) Curr Biol "Neuropeptide secreted from a pacemaker activates neurons to control a rhythmic ...."

(C) The expression pattern of snt-2. Representative fluorescent image of an adult expressing a snt-2 transcriptional reporter, in which GFP is driven by the snt-2 promoter fragment (4,177 bp, from 792 bp upstream of ATG to 33 bp of the second exon of snt-2 gene). Arrowheads indicate fluorescence in head and tail.

Picture from Gravato-Nobre MJ et al. (2011) Genetics "Glycosylation genes expressed in seam cells determine complex surface ...."

(D) Head region, showing seam-cell expression. (E) Tail region, showing weak intestinal expression. (F) Head region, showing expression in a few head cells, of uncertain identity. Scale bar: ~25 um.

Picture from Bretscher AJ et al. (2011) Neuron "Temperature, oxygen, and salt-sensing neurons in C. elegans are carbon dioxide ...."

cah-1::gfp labels ciliated head sensory neurons, head ganglia, the ventral nerve cord and tail neurons. Scale bar, 50 um.

Picture from Brozova E et al. (2006) Mech Dev "NHR-40, a Caenorhabditis elegans supplementary nuclear receptor, regulates ...."

Fig. 5. Expression of nhr-40::gfp regulated by promoter region 1. Promoter region 1 includes 2833 bp upstream of the initiation codon (ATG) of exon 1 of transcript nhr-40a and nhr-40b (construct #4586). Expression is first detected at the 1.5-fold stage of embryogenesis (A) and continues during larval stages to adulthood (B-D). Strong expression is observed in the anterior pharynx (arrow) and scattered head neurons (C) and in the rectal gland cells (D, arrow). Expression from truncated constructs #6011 (1013 bp) and #6059 (682 bp) is indistinguishable and is observed in neurons (E, axon marked by arrowhead, ventral neuronal cord marked by arrow) and uterine muscles (F) of young adults. Scale: 20 um.

Picture from Bretscher AJ et al. (2011) Neuron "Temperature, oxygen, and salt-sensing neurons in C. elegans are carbon dioxide ...."

cah-5::gfp labels head neurons and the intestine in L2 animals but neuronal expression in the head was absent by the young adult stage (not shown). Scale bar, 50 um.

Picture from Deplancke B et al. (2006) Cell "A gene-centered C. elegans protein-DNA interaction network."

(M) PZC395.8::GFP - GFP is expressed from late embryos to adults in the intestine, head muscles, dorsal nerve cord and two other neurons in the head close to the posterior pharyngeal bulb.

Picture from Van Buskirk C et al. (2010) Development "Paired and LIM class homeodomain proteins coordinate differentiation of the C. ...."

The head region of L4 stage animals showing des-2:GFP expression. In wild type, des-2:GFP is expressed in the M1 head muscles and several neurons, including ALA and RID.

Picture from Bretscher AJ et al. (2011) Neuron "Temperature, oxygen, and salt-sensing neurons in C. elegans are carbon dioxide ...."

cah-2::gfp labels a set of 4 quadrant neurons in the head, BAGL/R, other head neurons, the mid-body canal neurons CANL and CANR and tail neurons. Scale bar, 50 um.

Picture from Park JO et al. (2010) BMC Dev Biol "Characterization of the astacin family of metalloproteases in C. ...."

nas-31 expression. Side view of head region. Anterior is to the left.