Figure 6. Expression of
unc-14::gfp and
unc-51::gfp fusion genes in DD/VD and HSN neurons. Green represents GFP fluorescence, and red (Texas Red) represents neurons stained with an anti-GABA antibody or HSN neurons stained with an anti-serotonin antibody A Zeiss LSM410 confocal laser scanning microscope was used in these analyses. Bar, 50 hum. (A-F) Transgenic animals that express 1
unc-14::gfp promoter fusion gene. (A,D)
unc-14::GFP (green). (B,E) Staining with an antibody to GABA or serotonin (red). (C,F) Merge of A and B or D and E . The yellow color indicated by the arrows represents coexpression. (G-L) Transgenic animals that express an
unc-51::gfp promoter fusion gene. (G,J)
unc-51::GFP (green). (HK) Staining with an antibody to GABA or serotonin (red). (I,L) Merge of G and H or J and K. Yellow color indicated by arrowheads represents coexpression. The total number of cell bodies stained by the GABA antibody, including those in other focal planes, in a whole animal is -20. They include most of the 19 DD/VD neurons (McIntire et al. 1992), and most of them express
unc-14::GFP and
unc-51::GFP.