Picture from Hong Y et al. (1998) Development "Developmental regulation of a cyclin-dependent kinase inhibitor controls ...."

Figure 4. cki-1 5' upstream sequences control spatial and temporal expression patterns of cki-1::GFP. (A,C,E,G) maIs109 animals (transformed with pVT353, containing 2 kb of cki-1 upstream sequences driving GFP; see Materials and Methods). (B,D,F,H) maIs113 animals (transformed with pVT371, containing 8 kb of cki-1 upstream sequences). 2 kb (A) and 8 kb (B) constructs express GFP in resting Z1 and Z4 somatic gonad cells (arrowheads) in the early L1. The 2 kb (C) construct is not expressed, while the 8 kb construct (D) is expressed, in resting lateral hypodermal V cells (arrowheads) in the early L1. Both constructs (E,F) are expressed in distal tip cells (DTC, arrowheads), shown here at the L2 stage. The 2 kb construct (G) is not expressed, while the 8 kb construct (H) is expressed, in differentiating lateral hypodermal seam cells (arrowheads) at the L4 molt. Scale bar, 5 µm.

Picture from Banerjee D et al. (2005) Dev Cell "Developmental timing in C. elegans is regulated by kin-20 and tim-1, homologs of ...."

mRNA expression profiles of kin-20. A developmental Northern blot with total RNA from each developmental stage (E, embryos; L1-L4, larval stages 1-4; YA, young adult) was probed with a kin-20 fragment. Three signals were detected at all developmental stages (kin-20C at 4.0 kb, kin-20A at 2.5 kb, and kin-20B at 1.8 kb).

Picture from Harrington RJ et al. (2002) Development "The C. elegans LAR-like receptor tyrosine phosphatase PTP-3 and the VAB-1 Eph ...."

Northern blot of total C. elegans mRNA extracted from mixed-stage populations, probed with the PTP-3B cDNA (see Materials and Methods). Messages of approx. 8 kb and approx. 5 kb are detected.

Picture from Kawasaki I et al. (2007) Mech Dev "ASB-1, a germline-specific isoform of mitochondrial ATP synthase b subunit, is ...."

(A) asb-1 and asb-2 mRNA accumulation during development. RNA was prepared from wild-type hermaphrodite populations synchronized at each of the five different developmental stages (four larval and adult stages). asb-1 and asb-2 probes detected 1.3-kb and 1.6-kb transcripts, respectively. The 0.5-kb transcript of a ribosomal protein gene, rpp-1, was used as a loading control.

Picture from Huang TF et al. (2014) PLoS Genet "BLMP-1/Blimp-1 regulates the spatiotemporal cell migration pattern in C. ...."

Figure S4 Punc-5(1 kb)::gfp is expressed in DTCs during, and after, the dorsal turn. DIC and GFP images at different migration phases of DTCs in worms carrying the transgene Punc-5(1 kb)::gfp. DTCs are indicated by arrows.

Picture from Choi KY et al. (2003) Gene "Vacuolar-type H+-ATPase E subunit is required for embryogenesis and yolk ...."

(A) Northern blot analysis with 32P-labeled vha-8 probe detected two bands, 1.3 kb and 1.0 kb at all developmental stages: E, embryos; L2-L4, larval stages; A, adults. (B) Western blotting with anti-VHA-8 antibodies recognized a 29 kDa protein in wild-typeN2 worm lysate.

Picture from Yuan JY et al. (1992) Development "The Caenorhabditis elegans cell death gene ced-4 encodes a novel protein and is ...."

Figure 3. The ced-4 transcript is expressed mostly during embryogenesis. (a) A northern blot of poly(A)+ RNA from stage-synchronized animals was probed using pn1416, which hybridizes both to the 2.2 kb ced-4 transcript and to a 0.9 kb transcript (see Fig. 2A). Only the 2.2 kb transcript is shown in this figure. The 0.9 kb transcript is expressed mostly in eggs and adults (data not shown). (b) The presence of RNA in all lanes was confirmed by loading 1/10 of each sample on another gel and probing a northern blot from this gel using the C. elegans actin 1 gene (Krause and Hirsh, 1984).

Picture from Kaltenbach LS et al. (2000) Mol Cell "The TBP-like factor CeTLF is required to activate RNA polymerase II ...."

Figure 1. CeTLF Is Expressed Ubiquitously in Early Embryos. (A) Northern blots of early embryonic mRNA were probed for tlf-1 (left) or Cetbp (right; Lichtsteiner and Tjian 1993). The minor species below the tlf-1 band is not seen reproducibly (M. Horner and S. E. M., unpublished data). The open arrow denotes unspliced RNA, and the closed arrows denote tlf-1 (approximately 2.8 kb) and Cetbp (approximately 1.7 kb) mRNAs. 'M' refers to size markers.(B) Mouse embryonic Northern blot probed sequentially with a 1.5 kb fragment from mouse TLF (left) or TBP (right) cDNA. TLF produces two mRNAs of approximately 3.5 kb and 1.9 kb, indicated by arrows. TBP produces an mRNA of approximately 2.4 kb. 'dpc' denotes days post coitum. See Dantonel et al. 1999 for a sequence comparison of mouse and worm TLF.(C) Immunofluorescence for CeTLF (left) or CeTBP (right) in 2-cell (top) or 3-fold (middle) wild-type embryos. Bottom panels show CeTLF (left) and P granule (right) staining in a tlf-1(RNAi) embryo at approximately the 28-cell stage. Each embryo is 50 um.

Picture from Simon DJ et al. (2008) Cell "The microRNA miR-1 regulates a MEF-2-dependent retrograde signal at ...."

A transcriptional reporter containing a 3.7 kb mir-1 promoter driving expression of GFP showed expression in body-wall and pharyngeal muscles.

Picture from Ogawa H et al. (1998) J Biol Chem "Functional properties of the unc-64 gene encoding a Caenorhabditis elegans ...."

Figure 4. Northern analyses of poly(A)+ RNA probed with the C. elegans syntaxin cDNA. A, poly(A)+ RNA from asynchronous hermaphrodites.B, poly(A)+ RNA from developmentally staged hermaphrodites. Relative levels of the 1-kb RNA (empty circles) and 3-kb RNA (full circles) were determined by densitometric scanning of the autoradiographs.