Figure 3. Temporal expression of
pdi-3 during post-embryonic development assayed by semi-quantitative RT-PCR. The
pdi-3 transcript levels were compared with a constitutively expressed gene
ama-1. The mRNA was isolated from synchronous C. elegans cultures. Hours post-L1 larval arrest are indicated. The data was expressed as the ratio ofpdi-3 to
ama-1 transcripts. The RT-PCR was performed four independent times and the error bars indicate the mean +/- S.E.