Picture from Haklai-Topper L et al. (2010) Gene Expr Patterns "The neurexin superfamily of Caenorhabditis elegans."

Fig. 3. itx-1 expression. (A): itx-1prom::gfp expression in non-neuronal cells. (a) Intestinal expression in embryo and (b) in an L1 larva. (c) expression in spermatheca (white arrows) and (d) in vulva (white arrows). Blue star in (c) and (d) indicates gut expression. (B): itx-1prom::gfp expression in glial cells. (a) Expression in amphid socket cells; gfp signal is inverted for contrast; (b-e) co-labeling of itx-1-expressing sheath cells and sensory neurons filled with dye, as previously described (Perkins et al., 1986) (b), socket cells with itx-1prom::gfp (c), Normarksi image (d) and overlap of red and green in (e); (f): Sensory anatomy with associated sheath cell nuclei labeled in blue, socket cell nuclei in green and sensory neuron nuclei in red. Adapted from (Ward et al., 1975).

Picture from Haklai-Topper L et al. (2010) Gene Expr Patterns "The neurexin superfamily of Caenorhabditis elegans."

Fig. 5. ITX-1 protein localization. (A): ITX-1 localizes to adherence junctions in the intestine. anti-ITX-1 immunostaining of an L1 larval stage animal is shown. (B-C): Immuno-EM with anti-ITX-1 antibodies. Arrows point to the electron dense gold particles of ITX-1 antibody that is situated in close vicinity to the typical C. elegans adherens junction and along the baso-lateral membrane of intestinal cells. (D-F): ITX-1 antibody staining in the intestine, along the apical junction appears as a wider belt than the adherens junction marked using an antibody to AJM-1. (G-M): Co-localization of ITX-1 and b-G Spectrin in the intestine (G-J) and the pharynx (K-L). Panels F, I and M are merges of the preceding red and green images.

Picture from Haklai-Topper L et al. (2010) Gene Expr Patterns "The neurexin superfamily of Caenorhabditis elegans."

Fig. 2. nrx-1 expression and localization. (A): Expression of a gfp-tagged version of the genomic copy of the nrx-1 locus (see Fig. 1B for construct), expressed in transgenic animals. (a) Expression in a first larval stage animal. (b) Expression in head ganglia and nerve ring. (c) Expression in dorsal nerve cord and commissures. (d) Expression in ventral nerve cord with motor and interneuron. (e) Expression in ALM neuron. (f) Expression in neurons in tail ganglia. (B): Synaptic localization of NRX-1 protein. (a) Localization of gfp-tagged nrx-1, fused to the promoter of the sra-6 gene in the nerve ring of an adult wild type animal and in an unc-104 mutant animal (b). (c) Localization of bfp-tagged snt-1, fused to the promoter of the sra-6 gene in the nerve ring of an adult wild type animal and in an unc-104 mutant animal (d). (e) and (f) Overlap of panels above.

Picture from Haklai-Topper L et al. (2010) Gene Expr Patterns "The neurexin superfamily of Caenorhabditis elegans."

Fig. 4. nlr-1 expression. The head region of a transgenic young adult animal, expressing nlr-1prom::gfp is shown (see Fig. 1B for structure of reporter gene). No consistent or strong gfp expression is observed elsewhere in the worm. Expression is the same in all post-embryonic stages examined. Higher magnification of the boxed area is shown in the right panel.

Picture from Hao L et al. (2006) BMC Genomics "Comprehensive analysis of gene expression patterns of hedgehog-related ...."

(L) Pgrl-6::GFP is expressed in the PVT neuron.

Picture from Munro E et al. (2004) Dev Cell "Cortical flows powered by asymmetrical contraction transport PAR proteins to ...."

(J-L) Embryos fixed at metaphase and costained for NMY-2 and PAR-3.

Picture from Yin J et al. (2017) J Cell Biol "GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 in ...."

(J-L") Confocal fluorescence images in wild type coexpressing GFP::GOP-1and markers of different organelles including Cherry::RAB-5 (J-J", early endosome), Cherry::RAB-7 (K-K", late endosome), or NUC-1::Cherry (L-L", lysosome). White arrowheads, vesicles with overlapping GFP and Cherry fluorescence; pink arrowheads, GFP- and Cherry-positive vesicles that are in closeproximity; yellow arrowheads, GFP-positive and Cherry-negative puncta. Quantification is shown at right (mean +- SD). Bars: (A-I') 10 um; (J-L") 5 um.

Picture from Tian Y et al. (2010) Cell "C. elegans screen identifies autophagy genes specific to multicellular ...."

(G-L) The epg-4 reporter is expressed ubiquitously in embryos (H) and widely in larvae (I). EPG-4::GFP colocalizes with the ER marker cherry::TRAM-1 (J-L). (G) Nomarski image of the embryo in H.

Picture from Pujol N et al. (2001) J Biol Chem "The Caenorhabditis elegans unc-32 gene encodes alternative forms of a vacuolar ...."

In situ hybridization images on embryonic, larval, and adult worms with vha-6 (I-L) are shown. vha-6 is expressed only in the intestine, from the embryonic coma stage (I) through larvae (J and K) and adult (L).

Picture from Mahoney TR et al. (2008) Proc Natl Acad Sci U S A "Intestinal signaling to GABAergic neurons regulates a rhythmic behavior in ...."

(G'-L') aex-2 is expressed in the GABAergic neurons AVL and DVB as well as in enteric muscles. (G and I) AEX-2::mCherry is detected in the nerve ring (NR, arrow), ciliary sensory processes (CA, open arrow), nerve cord (I), and head mesodermal cell (HMC, arrowhead). (H) AEX-2::mCherry is expressed in the intestinal muscle (IM, arrow) and anal depressor (AD, arrowhead). (J'-L') mCherry expressed under the aex-2 promoter is detected in AVL (J-L, arrow) and DVB (J''-L'', arrow) GABAergic neurons. AEX-2::mCherry signal does not significantly overlap with GABAergic GFP in the ventral nerve cord (J'-L'). (Scale bar, 20 um.) See SI Text for transgenes and clones.