Figure 2.
osm-5::gfp is expressed in ciliated sensory neurons. Anatomical and ultrastructural features of C. elegans amphid and phasmid sensory neurons are depicted in the upper panels (Nomarski microscopy images) showing the adult hermaphrodite head, adult hermaphrodite tail, and ventral up adult male tail. Left is anterior; right is posterior. Middle panels are cartoons of amphid and phasmid sensory neurons, with magnification of the environmentally exposed cilia of the amphid and phasmid. Only one side is shown (for example, there are two PHA neurons, one left, and one right). Figures are reproduced from. OSM-5::GFP is localized in cell bodies ('cb'), dendrites ('d'), the transition zone ('tz'), and cilia ('c') of (a) the head, (b) the hermaphrodite tail, and (c) the male tail. In contrast, osm-5DeltaTPR::GFP is uniformly expressed throughout sensory neurons, is rarely seen in cilia, accumulates in the transition zone, and is observed in axons ('a') of (d) the head, (e) hermaphrodite tail, and (f) the male tail. Note the missing dendrites of one side of the phasmid neurons in (e)