Fig. 3. Photomicrographs of
ceh-38-GFP expression in C. elegans. Because of mosaic expression from arrays, not all cells are expressing GFP in each animal. GFP photomicrographs taken using a UV light source with a filterset appropriate for fluorescein isothiocyanate. (A) Embryo, DIC Nomarski photograph. (B) GFP expression in embryo corresponding to A. (C) GFP expression in embryo of similar stage as A. (D) DIC Nomarski photograph of coma-stage embryo and early two-fold embryo. (E) GFP expression in posterior part of coma-stage embryo in D; for comparison of GFP intensity, note the absence of staining in the early two-fold embryo, which presumbably lost the array. (F) GFP expression in the ring ganglion and the ventral nerve cord, and in the posterior gut. Gut and pharynx expression is seen in the L1 animal located on the right side. (G) Expression in L1 stage in many different tissues, i.e., gut, pharynx, hypodermis, neurons. (H) Expression of
ceh-38-GFP without NLS in axons of the ventral nerve cord (thin line along bottom of animal). Arrowhead: out-of-focus neuronal cell body. (I) Same as H, but different focal plain to show expression in cell body of ventral cord neuron. Expression in an anterior sector of the gut is also seen. Faint, small granules in F-H are gut granules which display autofluorescence.