Picture from Cui Y et al. (2009) J Biol Chem "Cadmium induces retinoic acid signaling by regulating retinoic acid metabolic ...."

Figure 1. bcmo-1::GFP expression in untreated C. elegans embryos. A, ~125 min post-fertilization; B, ~200 min post-fertilization; C, ~700 min post-fertilization. Embryos were obtained from nematodes not exposed to cadmium (magnification, 1200x).

Picture from Hobert O et al. (1999) J Cell Biol "A conserved LIM protein that affects muscular adherens junction integrity and ...."

(A-C): UNC-97::GFP localization in live worms at embryonic stage 300 min (A), 400 min (comma stage) (B), and 500 min (threefold pretzel stage) (C). (A'-C') Corresponding DIC micrographs. White arrows in C, a muscle nucleus and dense bodies, respectively. Note UNC-97::GFP localization to the periphery of nuclei in A-C.

Picture from Lin X et al. (2003) Curr Biol "C. elegans PAT-6/actopaxin plays a critical role in the assembly of integrin ...."

PAT-3 in wild-type 530 min embryos. Arrows indicate body wall muscle attachments.

Picture from Lin X et al. (2003) Curr Biol "C. elegans PAT-6/actopaxin plays a critical role in the assembly of integrin ...."

UNC-89 in wild-type 530 min embryos. Arrows indicate body wall muscle attachments.

Picture from Updike DL et al. (2006) PLoS Genet "Temporal regulation of foregut development by HTZ-1/H2A.Z and ...."

Expression of R07B1.9::GFP at 90 and 180 min after the comma stage. Pharyngeal R07B1.9::GFP is indicated by arrowheads.

Picture from Polley SR et al. (2014) Genetics "Implicating SCF complexes in organogenesis in Caenorhabditis elegans."

Figure 4 Embryonic expression pattern of SUP-36::GFP. (A-F) Confocal fluorescence images of full-length functional SUP- 36::GFP in embryos. A and B show embryos prior to morphogenesis (100-350 min postfertilization), C and D show embryos at early stages of morphogenesis (350-400 min), E shows an embryo at the 1.5-fold stage (430 min), and F shows an 3-fold-stage embryo (.600 min). Arrows in A, B, and E indicate the positions of nuclei. Arrowheads in C indicate expression of SUP-36:: GFP along the basal surface of primordial pharyngeal cells in the region abutting the basement membrane. Dotted outlined regions indicate the developing pharynx. Bar in A, 10 mm for A-F.

Picture from Sullivan-Brown JL et al. (2016) Genetics "Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube ...."

Figure 5 (A) Ea/Ep cell cycle times are reduced in some sptf-3 (RNAi) embryos. In wild-type embryos, the Ea cell cycle (left column) is 40.33 6 0.39 min, and the Ep cell cycle (right column) is 42.64 6 0.39 min. In sptf-3(RNAi) embryos, the cell cycle of the Ea and Ep cells is reduced to 37.28 6 0.65 and 39.51 6 0.57 min, respectively. Ea and Ep cell cycle timing is not changed in abi-1(RNAi), gex-3(RNAi), and ced-4 (RNAi) embryos. **P , 0.01. Excluded is one abi-1(RNAi) embryo that had an anomalous delay outside the range shown here (Ea = 62 min; Ep = 64 min). Cells that failed to internalize are shown in red. Gray indicates cells that internalized. Means are represented by thick black bars. (B) mNeonGreen::SPTF-3 is enriched in all nuclei during gastrulation (nine of nine embryos filmed). Arrowheads mark Ea/Ep nuclei. Bar, 10 um.

Picture from Chan SS-Y et al. (1996) Cell "UNC-40, a C. elegans homolog of DCC (Deleted in Colorectal Cancer), is required in ...."

Figure 4. Fluorescence Micrographs of UNC-40/GFP Expression in Transgenic Animals. (A-D) Embryos of successive stages. Anterior is shown to the right. (A) Early gastrula (100 min) showing ubiquitous GFP expression.(B) Longer exposure of late gastrula (260 min) showing decreased expression in the posterior embryo.(C) Neurula (430 min) showing expression in ventral cord motorneurons (between arrows).(D) Neurula (460 min) showing first axons from interneurons growing into the ventral nerve cord from the head (arrowhead).(E and F) Late-stage larvae. (E) Ventral aspect showing axons (arrowheads) of embryonic motorneurons DB4, DD3, and DD4. Additional motorneurons (not labeled) are visible alongside the main axon fascicle. (F) Lateral aspect showing sensory neurons PDEL and PVDL, motor axons (arrowheads), and excretory canal (ec). Scale bar, 10 um.

Picture from Peden E et al. (2007) Genes Dev "Control of sex-specific apoptosis in C. elegans by the BarH homeodomain protein ...."

Figure 4. CEH-30 expression patterns in C. elegans embryos. (A) GFP expression directed from an integrated array (smIs54) containing Pceh-30ceh-30::gfp at 360 min post-first cleavage. Three different focal planes of the same embryo are shown with the anterior toward the left and posterior toward the right. (B) Expression of CEH-30::GFP in a smIs54 XX embryo (420 min post-first cleavage, top panel) and a tra-1(e1099); smIs54 XX embryo at the similar stage (bottom panel). The ventral CEM neuron is indicated by a white arrowhead. (C) Frequency of CEH-30::GFP expression in ventral CEMs of XX embryos at 380-430 min after first cleavage. The genotypes of XX embryos are indicated.

Picture from Guse A et al. (2005) Curr Biol "Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of ...."

Figure 2. Phospho-S680 ZEN-4 Localizes to the Central Spindle in Anaphase and to Remnants in Late Cytokinesis, and a Nonphosphorylatable S680A Mutant of ZEN-4 Is Partially Functional In Vivo. (A) Localization of phospho-S680 ZEN-4 in C. elegans embryos was analyzed by immunofluorescence. The color images show the position of the fluorescence in relation to the spindle, and the monochrome images quantitatively show the signal intensity. ZEN-4 staining can be observed on the central spindle during anaphase and at remnants ([a] and [b], arrow; arrowhead). Phospho-S680 ZEN-4 staining can also be observed at the central spindle prior to furrow ingression ([a'], arrow) and at division remnants ([b'], arrowhead). To determine specificity of the phospho-CeMKLP1 antibody staining, we preincubated the antibody with either the phospho-peptide that the antibody was raised against or the corresponding non-phospho-peptide (7.4 μg/ml). Central spindle staining in anaphase persists after antibody preincubation with the non-phospho-peptide ([c], arrow) but not with the phospho-peptide ([d], arrow). Nonspecific centrosome staining persists after phospho-peptide incubation ([d], white arrowheads).(B) Simple, extrachromosomal transgenic arrays marked by rol-6(su1006) were generated in MG 376, which carries the zen-4 (w35) null allele, and the rescue efficiency was determined for five independent lines either expressing ZEN-4 WT or four lines expressing ZEN-4 S680A. Wild-type zen-4 rescues effectively the zen-4 null background with an efficiency of 69%, whereas the nonphosphorylatable zen-4 reduces the rescue efficiency to 18%. Error bars show the standard deviation. See Table S1 for details.(C) Complex extrachromosomal transgenic arrays marked by rol-6(su1006) were generated; these arrays rescue zen-4 (w35). ZEN-4::GFP localizes to the central spindle in anaphase (upper panel, 0 min) and localizes to the midbody and remnant in later stages of cytokinesis until sister-cell separation has been completed (upper panel, 6 min, 9 min, and 13 min). ZEN-4 S680A::GFP localizes to the central spindle in anaphase (lower panel, 0 min) and localizes to the midbody and early remnant (upper panel, 6 min and 9 min), but with furrow regression (upper panel, 9 min), ZEN-4 S680A::GFP moves with the regressing furrow (upper panel, 13 min) to the cortex (arrow).(D) Quantification of the frequency of failures in the first division of lines either expressing wild-type ZEN-4::GFP or ZEN-4 S680A::GFP.