Results of motility screens of C. elegans adults (A) in the wMicroTracker, B. pahangi females (B) & B. pahangi males (C) in both the WormAssay and the wMicroTracker, and B. pahangi microfilariae (D) , B. pahangi L3 (E) and S. mansoni schistosomules (F) in the wMicroTracker. Data are presented as mean +/- 95% CI.
Figure 5. RAB-6.2 associates with Golgi and GLR-1. (A and A') Cerulean:: RAB-6.2 (A) and MANS::YFP (A') in PVC cell bodies. (A'') Merged image showing Cerulean::RAB-6.2 colocalization to puncta with MANS::YFP (arrowheads). (B-C'') GLR-1::CFP (B and C) and Venus::RAB-6.2 (B' and C') in PVC cell bodies (B-B'') and dendrites (C-C''). (B'' and C'') Merged images show colocalization at specific points (arrowheads). (D-F) Subcellular localization of the indicated GFP::RAB-6.2 variant: wild type (D), GDP-locked mutant (E), and GTP-locked mutant (F). Bars, 5 um.
Figure 10. RAB-6.2 and LIN-10 colocalize with RME-8 in neurons. (A-D) Fluorescence from neuron cell bodies (A and C) and ventral cord dendrites (B and D) is shown. (A-B'') Cerulean::RAB-6.2 (A and B) colocalizes with Venus::RME-8 (A' and B'; A'' and B'' show merged). (C-D'') LIN-10::CFP (C and D) colocalizes with Venus::RME-8 (C' and D'; C'' and D'' show merged). Arrowheads indicate colabeled puncta. Bars, 5 um.
(B-E) smFISH using a lin-3 probe in C. elegans N2 (data from [2]) (B), C. briggsae AF16 (C), C. angaria RGD1 (D) and O. tipulae CEW1 (E). Red arrow marks the position of the anchor cell.
(B-C) An efn-2::GFP transcriptional reporter (Wang et al., 1999) shows GFP expression in hyp7 (arrowhead) during the time of DTC migration (B), but not before DTC migration (C). The bright spots in panel C are nonspecific gut autofluorescence.