(A) Single-plane confocal images of wild-type and
pkc-3 (RNAi) embryos expressing GFP::PAR-1 or Dendra::MEX-5. Note that in
pkc-3(RNAi) embryos, PAR-1 localizes throughout the cortex and cytoplasm and DendraR::MEX-5 is evenly distributed throughout the cytoplasm. PAR-1 also localizes on centrosomes (bright dots) as reported previously (Gnczy et al., 2001). (B) Single-plane confocal images of zygotes of the indicated genotypes immunostained with antibodies against PKC-3 (Aono et al., 2004) and PAR-1 (Guo and Kemphues, 1995) or expressing Dendra::MEX-5. PKC-3 is enriched on the anterior cortex in all genotypes.